GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.0.5 mg/mL
ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
Host species
Rabbit
Specificity
CD3 zeta
Immunogen
A KLH conjugated synthetic peptide from the C-terminal region of human CD3Z (AA 113-141)
Positive Control
WB: Jurkat, MOLT-4 and CTLL-2 cell lysates. Flow: K562 cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified + Peptide affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
CD3 zeta Antibody (Ab094480) - Western Blot All lanes: CD3 zeta Antibody (Ab094480) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 19 kDa Observed band size: 16 kDa Exposure time: 52.9 s
CD3 zeta Antibody (Ab094480) - Flow Cytometry Overlay histogram showing K562 cells stained with CD3 zeta Antibody (Ab094480)(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the CD3 zeta Antibody (Ab094480)(1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
Application
Application
Dilution info
Flow Cytometry
1/25
WB
1/4000-1/8000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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