Osteopontin Antibody - Primary antibody, specific to Osteopontin, Rabbit IgG

Cat. No.: Ab119430
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. See COA
Application
IHC, WB
Host species
Rabbit
Reactive species
Human, Mouse, Pig
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab119430-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$99.90
50μl
Ab119430-50μl
4
$259.90
100μl
Ab119430-100μl
3
$419.90
1ml
Ab119430-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human Osteopontin Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.01-3 µg/mL IHC: 5-30 µg/mL

Protein Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Specifications

Product Name
Osteopontin Antibody - Primary antibody, specific to Osteopontin, Rabbit IgG
Synonyms
BNSP antibody | Bone sialoprotein 1 antibody | Bone sialoprotein I antibody | BSP I antibody | BSPI antibody | Early T lymphocyte activation 1 antibody | ETA 1 antibody | ETA1 antibody | MGC110940 antibody | Nephropontin antibody | OPN antibody | Osteopon
Specifications & Purity
Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species
Rabbit
Specificity
Osteopontin
Immunogen
Recombinant Osteopontin expressed in E.coli (AA 17-287).
Positive Control
WB: A549, Hep G2, HEK293, RAW 264.7 and NIH/3T3 cell lysates. IHC: Human kidney tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified + Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Osteopontin Antibody (Ab119430) - Western Blot
All lanes: Osteopontin Antibody (Ab119430) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 32, 45 kDa
Exposure time: 15.6 s 

Osteopontin Antibody (Ab119430) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Osteopontin Antibody (Ab119430)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Application
ApplicationDilution info
IHC

5-30 µg/mL

WB

0.01-3 µg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
SPP1 Tbio Osteopontin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101832Certificate of AnalysisNov 27, 2023 Ab119430
ZJ23F1101814Certificate of AnalysisNov 27, 2023 Ab119430
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →

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