GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Recombinant, 0.5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ATG4C Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Specifications
Product Name
Recombinant ATG4C Antibody - Primary antibody, specific to ATG4C, Rabbit IgG
Synonyms
APG4 autophagy 4 homolog C (S. cerevisiae) antibody | APG4 autophagy 4 homolog C antibody | APG4 C antibody | APG4-C antibody | APG4C antibody | ATG 4C antibody | ATG4 autophagy related 4 homolog C (S. cerevisiae) antibody | ATG4 autophagy related 4 homol
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species
Rabbit
Specificity
ATG4C
Immunogen
Recombinant protein of human ATG4C (AA 354-454)
Positive Control
WB: K562, HeLa, Jurkat, Hep G2 and Raji cell lysates. IHC: Human lung tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ATG4C Antibody (Ab090012) - Western Blot All lanes: Recombinant ATG4C Antibody (Ab090012) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 4.6 s
Recombinant ATG4C Antibody (Ab090012) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant ATG4C Antibody (Ab090012). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant ATG4C Antibody (Ab090012) - IF/ICC IF analysis of Recombinant ATG4C Antibody (Ab090012) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant ATG4C Antibody (Ab090012) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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