GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Estrogen Receptor alpha Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant Estrogen Receptor alpha Antibody - Primary antibody, specific to Estrogen Receptor alpha, Rabbit IgG
Recombinant, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Host species
Rabbit
Specificity
Estrogen Receptor alpha
Immunogen
A synthetic peptide derived from human Estrogen Receptor alpha (AA 98-116)
Positive Control
WB: MCF7 and NIH/3T3 cell lysates. IHC: Human breast cancer tissue. ICC/IF: MCF7 cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Estrogen Receptor alpha Antibody (
Ab102356
) - Western Blot All lanes: Recombinant Estrogen Receptor alpha Antibody (Ab102356) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 30.2 s
Recombinant Estrogen Receptor alpha Antibody (Ab102356) - IF/ICC Immunocytochemistry analysis of Estrogen Receptor alpha (green) in MCF7 using Recombinant Estrogen Receptor alpha Antibody (Ab102356), and cells were counterstained with DAPI (blue).
Recombinant Estrogen Receptor alpha Antibody (Ab102356) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Estrogen Receptor alpha Antibody (Ab102356). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/1000
IF/ICC
1/100 - 1/200
IHC
1/200-1/20000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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