GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Protein Function： Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor.
Specifications
Product Name
Recombinant IRF1 Antibody - Primary antibody, specific to IRF1, Rabbit IgG
WB: K562, MCF7, Jurkat, THP-1, MOLT-4, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: U87-MG cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Note
This product is about to be sold out and removed from the shelves. The replacement product is Ab111193.
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant IRF1 Antibody (Ab111190) - Western Blot All lanes: Recombinant IRF1 Antibody (Ab111190) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa Observed band size: 50 kDa Exposure time: 94.1 s
Recombinant IRF1 Antibody (Ab111190) - IF/ICC Immunocytochemistry analysis of IRF1 (green) in U87-MG using Recombinant IRF1 Antibody (Ab111190), and cells were counterstained with DAPI (blue).
Recombinant IRF1 Antibody (Ab111190) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant IRF1 Antibody (Ab111190). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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