GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.6 mg/mL
ExactAb™, Validated, Recombinant, 0.6 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human LINE-1 ORF1p Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 Relevance Domain: The coiled coil domain mediates homotrimerization. The RRM and the CTD domain are both required for proper RNA-binding activity. Function: Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. Long interspersed element-1/LINE-1/L1 retrotransposons are present in more than 500'000 full (6 kb) or truncated copies in the human genome. Most of them are inactive but 80 to 100 of those elements could be transcribed, translated and active in any individual. An active LINE-1 encodes for 2 proteins translated from a single RNA containing 2 non-overlapping ORFs, ORF1 and ORF2. ORF1p is described in this entry as a representative of all ORF1p potentially expressed by active elements. ORF2p is described in the related entry AC O00370. PTM: Polyubiquitinated, probably by UBR2, which induces its degradation. Similarity: Belongs to the transposase 22 family.
Specifications
Product Name
Recombinant LINE-1 ORF1p Antibody - Primary antibody, specific to L1RE1, Rabbit IgG
Synonyms
L1ORF1p antibody | L1RE1 antibody | LINE retrotransposable element 1 antibody | LINE-1 retrotransposable element ORF1 protein antibody | LINE1 retrotransposable element 1 antibody | LRE1 antibody | L1RE1 | L1ORF1p | LRE1 | LINE-1 ORF1p antibody | Anti-LIN
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species
Rabbit
Specificity
L1RE1
Immunogen
Recombinant protein of human LINE-1 ORF1p (AA 157-330)
Positive Control
WB: HeLa, A431, U-2 OS and A549 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.6 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant LINE-1 ORF1p Antibody (Ab113189) - Western Blot All lanes: Recombinant LINE-1 ORF1p Antibody (Ab113189) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 39 kDa Exposure time: 56.9 s
Recombinant LINE-1 ORF1p Antibody (Ab113189) - Western Blot All lanes: Recombinant LINE-1 ORF1p Antibody (Ab113189) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 10.0 s
Application
Application
Dilution info
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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