Recombinant NF-kB p65 Antibody - Primary antibody, specific to RELA, Rabbit IgG

Cat. No.: Ab117948

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human NF-kB p65 Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/2000

IHC: 1/50-1/100

IF/ICC: 1/200

Protein Function

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Specifications

Product Name

Recombinant NF-kB p65 Antibody - Primary antibody, specific to RELA, Rabbit IgG

Synonyms

NFKB3 | RELA | TF65 | Transcription factor p65 | p65 | NFkB | NF-KB p65 Rabbit mAb | Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody | MGC131774 antibody | NF kappa B p65delta3 antibody | nfkappabp65 antibody | NFkB p65 antibody | NF

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.6 mg/mL

Host species

Rabbit

Specificity

RELA

Immunogen

A synthetic peptide derived from human NF-kB p65 (AA 506-524).

Positive Control

WB: K562, NIH/3T3, HeLa, MCF7 and Jurkat cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

0.6 mg/mL

Note

This product is nearing end-of-life and will be discontinued. Replacement product: Ab117947

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant NF-kB p65 Antibody (Ab117948)

Recombinant NF-kB p65 Antibody (Ab117948) - Western Blot
All lanes: Recombinant NF-kB p65 Antibody (Ab117948) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 1.8 s

IHC: Recombinant NF-kB p65 Antibody (Ab117948)

Recombinant NF-kB p65 Antibody (Ab117948) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NF-kB p65 Antibody (Ab117948). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant NF-kB p65 Antibody (Ab117948)

Recombinant NF-kB p65 Antibody (Ab117948) - IF/ICC

IF analysis of Recombinant NF-kB p65 Antibody (Ab117948) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant NF-kB p65 Antibody (Ab117948) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

RELA Tchem Transcription factor p65 (0 Activities)

Discover Target: RELA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action