Recombinant SF3B1 Antibody - Primary antibody, specific to SF3B1, Rabbit IgG

Cat. No.: Ab127273
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.2 mg/mL
Application
IF/ICC, IHC, WB
Host species
Rabbit
Reactive species
Hamster, Human, Mouse, Rat
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab127273-10μl
2
$99.90
50μl
Ab127273-50μl
1
$259.90
100μl
Ab127273-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab127273-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human SF3B1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Relevance:
SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.

Specifications

Product Name
Recombinant SF3B1 Antibody - Primary antibody, specific to SF3B1, Rabbit IgG
Synonyms
Pre-mRNA-splicing factor SF3b 155 kDa subunit | SAP 155 | SF3b155 | Spliceosome-associated protein 155 | Hsh 155 antibody | MDS antibody | Pre mRNA processing 10 antibody | Pre mRNA splicing factor SF3b 155 kDa subunitantibody | PRP 10 antibody | PRP10 an
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species
Rabbit
Specificity
SF3B1
Immunogen
A synthetic peptide derived from human SF3B1 (AA 21-34)
Positive Control
WB: HeLa, Ramos, K562, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: 293T cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.2 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant SF3B1 Antibody (Ab127273) - Western Blot
All lanes: Recombinant SF3B1 Antibody (Ab127273) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 146 kDa
Observed band size: 146 kDa
Exposure time: 2.8 s

Recombinant SF3B1 Antibody (Ab127273) - IF/ICC
Immunocytochemistry analysis of SF3B1 (green) in 293T using Recombinant SF3B1 Antibody (Ab127273), and cells were counterstained with DAPI (blue).

Recombinant SF3B1 Antibody (Ab127273) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant SF3B1 Antibody (Ab127273). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application
ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
SF3B1 Tbio Splicing factor 3B subunit 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0403983Certificate of AnalysisApr 01, 2024 Ab127273
ZJ24F0403982Certificate of AnalysisApr 01, 2024 Ab127273
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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