GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, Recombinant, ExactAb™, KD Validation, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitinat
Host species
Rabbit
Specificity
NEDD4L
Immunogen
A synthesized peptide derived from human NEDD4-2 (AA 480-602).
Carrier Free, ExactAb™, High Performance, KD Validation, Recombinant, Validated
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NEDD4L Antibody (Ab327032) - Western Blot
All lanes: Recombinant NEDD4L Antibody (Ab327032) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 119 kDa
Exposure time: 47.2 s
Recombinant NEDD4L Antibody (Ab327032) - Western Blot
Western blotting analysis using Recombinant NEDD4L Antibody (Ab327032). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Recombinant NEDD4L Antibody (Ab327032) (1/20000) and Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) (1/20000) respectively.
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