GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.3 mg/mL
Protein Function Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
Specifications
Product Name
Recombinant NXF1 Antibody - Primary antibody, specific to NXF1, Rabbit IgG
A synthetic peptide derived from human NXF1 (AA 4-17)
Positive Control
WB: K562, HeLa, HEK293, Hep G2 and A549 cell lysates. IHC: Human breast cancer tissue. ICC/IF: Jurkat cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.3 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NXF1 Antibody (Ab119077) - Western Blot All lanes: Recombinant NXF1 Antibody (Ab119077) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 57.6 s
Recombinant NXF1 Antibody (Ab119077) - IF/ICC Immunocytochemistry analysis of NXF1 (green) in Jurkat using Recombinant NXF1 Antibody (Ab119077), and cells were counterstained with DAPI (blue).
Recombinant NXF1 Antibody (Ab119077) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant NXF1 Antibody (Ab119077). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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