GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.7 mg/mL
ExactAb™, Validated, Recombinant, 0.7 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Ube2N / Ubc13 Antibody, Recombinant, could be used for WB and so on. Application · WB: 1/500-1/1000 Protein Function The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
Specifications
Product Name
Recombinant Ube2N/Ubc13 Antibody - Primary antibody, specific to UBE2N, Rabbit IgG
Synonyms
Bendless-like ubiquitin-conjugating enzyme | E2 ubiquitin-conjugating enzyme N | EC 2.3.2.23 | Ubc13 | UbcH13 | Ubiquitin carrier protein N | Ubiquitin-protein ligase N | Bendless like ubiquitin conjugating enzyme antibody | Bendless-like ubiquitin-conjug
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species
Rabbit
Specificity
UBE2N
Immunogen
A synthetic peptide derived from human Ube2N/Ubc13 (AA 1-50)
Positive Control
WB: Jurkat, Raji, HEK293, U2OS, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
0.7 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Ube2N/Ubc13 Antibody (Ab133260) - Western Blot All lanes: Recombinant Ube2N/Ubc13 Antibody (Ab133260) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa Observed band size: 16 kDa Exposure time: 63.9 s
Application
Application
Dilution info
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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