After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
posted this at Jun 9, 2025 Updated at Aug 28, 2024
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After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
The blistering of cell membrane may be caused by too long fixation time, you can shorten the fixation time appropriately to optimize the fixation result.
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