Isoform controls help eliminate nonspecific staining, so how do you set the door?

Isoform controls help eliminate nonspecific staining, so how do you set the door?

We suggested using FMO combined isotype control as a negative control to go through the trap door, but FMO combined isotype control is more complicated, so many experimenters choose a simple method - using only isotype control as a negative control to go through the trap door. The isotype control is used to eliminate background staining due to non-specific binding of antibodies to help find true negative cell populations.

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