GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Calpain 2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application: WB: 0.01-2 µg/mL IHC: 5-20 µg/mL ICC/IF: 5-20 µg/mL Protein Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Specifications
Product Name
Calpain 2 Antibody - Primary antibody, specific to Calpain 2, Rabbit IgG
Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species
Rabbit
Specificity
Calpain 2
Immunogen
Recombinant CAPN2 expressed in E.coli (AA 45-514).
Positive Control
WB: HeLa, A549 and HEK293 cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified + Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Calpain 2 Antibody (Ab092535) - Western Blot All lanes: Calpain 2 Antibody (Ab092535) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 80 kDa Observed band size: 76 kDa Exposure time: 52.9 s
Calpain 2 Antibody (Ab092535) - IF/ICC FITC staining on IF Samples: Human HeLa Cells. Primary Ab: 10 µg/mL Calpain 2 Antibody (Ab092535).
Calpain 2 Antibody (Ab092535) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL Calpain 2 Antibody (Ab092535).
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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