p38 gamma/MAPK12 Antibody - Primary antibody, specific to MAPK12, Rabbit IgG

Cat. No.: Ab119728

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.4 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human p38 gamma/MAPK12 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.

Specifications

Product Name

p38 gamma/MAPK12 Antibody - Primary antibody, specific to MAPK12, Rabbit IgG

Synonyms

EC 2.7.11 | ERK3 | ERK-6 | ERK6 | MAPK 12 | P38GAMMA | Extracellular signal-regulated kinase 6 | MAP kinase 12 | MAP kinase p38 gamma | MAPK12 | mitogen-activated protein kinase 12 | mitogen-activated protein kinase 3 | Mitogen-activated protein kinase p3

Specifications & Purity

ExactAb™, Validated, Carrier Free, 0.4 mg/mL

Host species

Rabbit

Specificity

MAPK12

Immunogen

A synthetic peptide derived from human MAPK12 (AA 106-131).

Positive Control

WB: THP-1, K562 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

0.4 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: p38 gamma/MAPK12 Antibody (Ab119728)

p38 gamma/MAPK12 Antibody (Ab119728) - Western Blot
All lanes: p38 gamma/MAPK12 Antibody (Ab119728) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 150.0 s

IHC: p38 gamma/MAPK12 Antibody (Ab119728)

p38 gamma/MAPK12 Antibody (Ab119728) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p38 gamma/MAPK12 Antibody (Ab119728). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: p38 gamma/MAPK12 Antibody (Ab119728)

p38 gamma/MAPK12 Antibody (Ab119728) - IF/ICC

IF analysis of p38 gamma/MAPK12 Antibody (Ab119728) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with p38 gamma/MAPK12 Antibody (Ab119728) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

MAPK12 Tchem Mitogen-activated protein kinase 12 (0 Activities)

Discover Target: MAPK12

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

References

1. Peng Zhu, Jianqiang Qian, Zhongyuan Xu, Chi Meng, Ji Liu, Wenpei Shan, Weizhong Zhu, Yongjun Wang, Yumin Yang, Wei Zhang, Yanan Zhang, Yong Ling.  (2020)  Piperlonguminine and Piperine Analogues as TrxR Inhibitors that Promote ROS and Autophagy and Regulate p38 and Akt/mTOR Signaling.  JOURNAL OF NATURAL PRODUCTS,      [PMID:33026807] [10.1021/acs.jnatprod.0c00599]