ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human MCM3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
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Protein Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR.
Recombinant MCM3 Antibody (Ab114541) - Western Blot
All lanes: Recombinant MCM3 Antibody (Ab114541) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 97 kDa
Exposure time: 11.6 s
Recombinant MCM3 Antibody (Ab114541) - IF/ICC
Immunocytochemistry analysis of MCM3 (green) in HeLa using Recombinant MCM3 Antibody (Ab114541), and cells were counterstained with DAPI (blue).
Recombinant MCM3 Antibody (Ab114541) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM3 Antibody (Ab114541). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303901 | Certificate of Analysis | Apr 02, 2024 | Ab114541 |
| ZJ24F0303900 | Certificate of Analysis | Apr 02, 2024 | Ab114541 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →
