GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Ubiquitin Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant Ubiquitin Antibody - Primary antibody, specific to Ubiquitin, Rabbit IgG
Synonyms
HEL-S-50 | UBB | Ubiquitin/Ubiquitin+1 | Epididymis secretory protein Li 50 antibody | FLJ25987 antibody | HEL S 50 antibody | MGC8385 antibody | Polyubiquitin B antibody | RPS 27A antibody | RPS27A antibody | UBA 52 antibody | UBA 80 antibody | UBA52 ant
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the
Host species
Rabbit
Specificity
Ubiquitin
Immunogen
A synthetic peptide derived from human Ubiquitin (AA 50-76)
Positive Control
WB: Mouse brain, Rat brain, Hep G2, HeLa, Jurkat, MCF7, SH-SY5Y and HEK293 lysates. IHC: Human breast cancer tissue. IF/ICC: Jurkat cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Ubiquitin Antibody (Ab133323) - Western Blot All lanes: Recombinant Ubiquitin Antibody (Ab133323) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 10 kDa Exposure time: 106.6 s
Recombinant Ubiquitin Antibody (Ab133323) - IHC Immunohistochemical analysis of paraffin-embedded human breast cancer, using Recombinant Ubiquitin Antibody (Ab133323).
Recombinant Ubiquitin Antibody (Ab133323) - IF/ICC Immunofluorescent analysis of Jurkat cells, using Recombinant Ubiquitin Antibody (Ab133323).
Application
Application
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50 - 1/200
WB
1/1000-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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