Recombinant AMPK gamma 1 Antibody - Primary antibody, specific to AMPK gamma 1, Rabbit IgG

Cat. No.: Ab088680
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
IF/ICC, WB
Host species
Rabbit
Reactive species
Human
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab088680-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$99.90
50μl
Ab088680-50μl
1
$259.90
100μl
Ab088680-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab088680-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 0.5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human AMPK gamma 1 Antibody, Recombinant, could be used for WB, IF, ICC and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function:
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Specifications

Product Name
Recombinant AMPK gamma 1 Antibody - Primary antibody, specific to AMPK gamma 1, Rabbit IgG
Synonyms
5' AMP activated protein kinasegamma 1 subunit antibody | 5' AMP activated protein kinase subunit gamma 1 antibody | 5''-AMP-activated protein kinase subunit gamma-1 antibody | AAKG1_HUMAN antibody | AMP activated protein kinase noncatalytic gamma 1 subun
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species
Rabbit
Specificity
AMPK gamma 1
Immunogen
A synthetic peptide derived from human AMPK gamma 1 (AA 1-19)
Positive Control
WB: HeLa, A549, HL-60, U20S lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purification
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant AMPK gamma 1 Antibody (Ab088680) - Western Blot
All lanes: Recombinant AMPK gamma 1 Antibody (Ab088680) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 36 kDa
Exposure time: 24.7 s

Recombinant AMPK gamma 1 Antibody (Ab088680) - IF/ICC
Immunocytochemistry analysis of AMPK gamma 1 (green) in HeLa using Recombinant AMPK gamma 1 Antibody (Ab088680), and cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
PRKAG1 Tchem 5'-AMP-activated protein kinase subunit gamma-1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PRKAG1 Tchem AMP-activated protein kinase, gamma-1 subunit (254 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202192Certificate of AnalysisDec 28, 2023 Ab088680
ZJ23F1202191Certificate of AnalysisDec 28, 2023 Ab088680
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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