Recombinant CD16/CD32 Antibody (Biotin) - Primary antibody, specific to CD16/32

Cat. No.: Ab213312

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P05C29

Conjugation

Biotin

Overview

Recombinant CD16/CD32 Antibody, clone P05C29, could be used for Flow Cytometry and so on.

Specifications

Product Name

Recombinant CD16/CD32 Antibody (Biotin) - Primary antibody, specific to CD16/32

Synonyms

CD32 | Fcgr2 | Fcr-2 | Fcr-3 | Ly-17 | LyM-1 | fcRII | FcgRII | Fcgr2a | Ly-m20 | Fc[g]RII | F630109E10Rik | CD16 | CD16A | CD16-II | FCG3 | FCGR3 | FCGR3A | FCGRIII | FcGRIIIA | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | Low affinity immunoglobulin gamm

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

CD16 and CD32 serve as low affinity receptors for IgG Fc constant regions and are involved in regulating various cellular functions including antibody-dependent cellular toxicity (ADCC), phagocytosis, effector cell degranulation, and B cell proliferation.

Host species

Rat

Specificity

CD16/32

Positive Control

Flow: C57BL/6 mouse splenocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD16/CD32 Antibody (Biotin) (Ab213312)

Recombinant CD16/CD32 Antibody (Biotin) (Ab213312) - Flow Cytometry
Two-color flow cytometric analysis of CD16/CD32 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (Biotin) (Ab180310; Left panel) or Recombinant CD16/CD32 Antibody (Biotin) (Ab213312; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.
 

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

FCGR2B Tbio Low affinity immunoglobulin gamma Fc region receptor II-b (0 Activities)

Discover Target: FCGR2B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)