Emicizumab (anti-Factor IX) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to F9, >95%, Human IgG4SP, Antibody of coagulation factor IX;Antibody of coagulation factor X, Antibody of coagulation factor IX;Antibody of coagulation factor X

CAS: 1610943-06-0 Cat. No.: Ab170499

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human

Isotype

Human IgG4SP

Conjugation

Unconjugated

Overview

Emicizumab is a bispecific monoclonal antibody that bridges activated factor IX and factor X to replace the function of missing activated factor VIII, thereby restoring hemostasis. Emicizumab can be used for hemophilia A research.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Specifications

Product Name

Emicizumab (anti-Factor IX) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to F9, >95%, Human IgG4SP, Antibody of coagulation factor IX;Antibody of coagulation factor X

Synonyms

Christmas Disease antibody | Christmas factor antibody | Coagulant factor IX antibody | Coagulation factor 9 antibody | Coagulation factor IX antibody | Coagulation factor IXa heavy chain antibody | F9 antibody | FA9_HUMAN antibody | Factor 9 antibody | F

Specifications & Purity

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Host species

Human

Specificity

F9

Application

ELISA, Functional Assay, Flow cytometry, Kinetics (BLI), Kinetics (SPR)

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Action Type

OTHER

Mechanism of action

Antibody of coagulation factor IX;Antibody of coagulation factor X

Antibody Type

Primary antibody

Clonality

Recombinant

Isotype

Human IgG4SP

Light Chain Type

kappa

SDS-PAGE

27.0 kDa (Light Chain) & 52.4 kDa (Heavy Chain), under reducing conditions; 182.7 kDa, under non-reducing conditions.

Purification Method

Protein A purified

Purity

>95%

Source

CHO supernatant

Concentration

See COA

CAS

1610943-06-0

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: Emicizumab (anti-Factor IX) (Ab170499)

Emicizumab (anti-Factor IX) (Ab170499) - Flow Cytometry
Flow Cytometry analysis of HEK293 cells labelling Factor IX (red) with Emicizumab (anti-Factor IX) (Ab170499). The cells were fixed and permeabilized with methanol (5 min). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Emicizumab (anti-Factor IX) (Ab170499)

Emicizumab (anti-Factor IX) (Ab170499) - SEC
The purity of Emicizumab (anti-Factor IX) (Ab170499) is more than 95% verified by HPLC.

Associated Targets(Human)

F10 Tclin Coagulation factor X (0 Activities)

Discover Target: F10

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

F9 Tchem Coagulation factor IX (0 Activities)

Discover Target: F9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

F9 Tchem Coagulation factor IX (922 Activities)

Discover Target: F9

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

F10 Tclin Coagulation factor X (9693 Activities)

Discover Target: F10

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

F10 Tclin Coagulation factor VII and X (116 Activities)

Discover Target: F10

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

F9 Tchem Coagulation factor IX and X (91 Activities)

Discover Target: F9

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action