Recombinant c-Maf Antibody - Primary antibody, specific to MAF, Rabbit IgG

Cat. No.: Ab096954

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human c-Maf Antibody, Recombinant, could be used for WB and so on.
Protein Function
Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glukagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glukagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant reponse element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
Post-translational
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by glucocorticoids. Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable). The phosphorylation status can serve to either stimulate or inhibit transcription.

Specifications

Product Name

Recombinant c-Maf Antibody - Primary antibody, specific to MAF, Rabbit IgG

Synonyms

Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene antibody | Avian musculoaponeurotic fibrosarcoma (v maf)antibody | c maf proto oncogene antibody | cMaf antibody | maf antibody | MAF_HUMAN antibody | MAF2 antibody | MGC71685 antibody | AS42 oncog

Specifications & Purity

Recombinant, ExactAb™, Validated, See COA

Host species

Rabbit

Specificity

MAF

Immunogen

A synthetic peptide derived from human c-Maf (AA 331-362)

Positive Control

WB: HeLa, Jurkat, Hep G2, A549, HUVEC, Raji, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant c-Maf Antibody (Ab096954)

Recombinant c-Maf Antibody (Ab096954) - Western Blot
All lanes: Recombinant c-Maf Antibody (Ab096954) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 32.9 s

Application

• Application: Dilution info
• WB:

  1/500-1/2000 ;

Associated Targets(Human)

MAF Tbio Transcription factor Maf (0 Activities)

Discover Target: MAF

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)