GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human AMPK alpha 1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant AMPK alpha 1 Antibody - Primary antibody, specific to PRKAA1, Rabbit IgG
Synonyms
5 AMP activated protein kinase alpha 1catalytic subunit antibody | 5 AMP activated protein kinase catalytic alpha 1 chain antibody | 5' AMP activated protein kinase catalytic subunit alpha 1 antibody | 5'-AMP-activated protein kinase catalytic subunit alp
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears
Host species
Rabbit
Specificity
PRKAA1
Immunogen
A synthetic peptide derived from human AMPK alpha 1 (AA 500-559).
Positive Control
WB: Raw264.7, MCF7, C6, K562, Hep G2, NIH/3T3 and HeLa cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant AMPK alpha 1 Antibody (Ab088655) - Western Blot All lanes: Recombinant AMPK alpha 1 Antibody (Ab088655) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 66 kDa Exposure time: 5.0 s
Recombinant AMPK alpha 1 Antibody (Ab088655) - IF/ICC Immunocytochemistry analysis of AMPK alpha 1 (green) in HeLa cells using Recombinant AMPK alpha 1 Antibody (Ab088655), and cells were counterstained with DAPI (blue).
Recombinant AMPK alpha 1 Antibody (Ab088655) - IHC Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Recombinant AMPK alpha 1 Antibody (Ab088655). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0404137
