Recombinant JNK2 Antibody - Primary antibody, specific to JNK2, Rabbit IgG

Cat. No.: Ab111533

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.3 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human JNK2 Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB:  1/500-1/1000       

IHC:  1/50-1/100

IF/ICC: 1/200

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Specifications

Product Name

Recombinant JNK2 Antibody - Primary antibody, specific to JNK2, Rabbit IgG

Synonyms

c Jun kinase 2 antibody | C Jun N terminal kinase 2 antibody | c-Jun N-terminal kinase 2 antibody | JNK 55 antibody | JNK-55 antibody | JNK2 alpha antibody | JNK2 antibody | JNK2 beta antibody | JNK2A antibody | JNK2alpha antibody | JNK2B antibody | JNK2B

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.3 mg/mL

Host species

Rabbit

Specificity

JNK2

Immunogen

A synthetic peptide derived from human JNK2 (AA 361-374).

Positive Control

WB: K562, HEK293, C6 and NIH/3T3 cell lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Antigen affinity purified

Concentration

0.3 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant JNK2 Antibody (Ab111533)

Recombinant JNK2 Antibody (Ab111533) - Western Blot
All lanes: Recombinant JNK2 Antibody (Ab111533) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 41, 49 kDa
Exposure time: 6.4 s

IHC: Recombinant JNK2 Antibody (Ab111533)

Recombinant JNK2 Antibody (Ab111533) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant JNK2 Antibody (Ab111533). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant JNK2 Antibody (Ab111533)

Recombinant JNK2 Antibody (Ab111533) - IF/ICC

 IF analysis of Recombinant JNK2 Antibody (Ab111533) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant JNK2 Antibody (Ab111533) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

MAPK9 Tchem Mitogen-activated protein kinase 9 (0 Activities)

Discover Target: MAPK9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action