GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.3 mg/mL
ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human MCM7/PRL Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Specifications
Product Name
Recombinant MCM7/PRL Antibody - Primary antibody, specific to MCM7, Rabbit IgG
Synonyms
CDABP0042 antibody | CDC 47 antibody | CDC47 antibody | CDC47 homolog antibody | Cdc47, S. cerevisiae, homolog of antibody | DNA replication licensing factor MCM7 antibody | Homolog of S. cerevisiae Cdc47 antibody | MCM 2 antibody | MCM 7 antibody | MCM2
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species
Rabbit
Specificity
MCM7
Immunogen
A synthetic peptide derived from human MCM7 (AA 671-683).
Positive Control
WB: MOLT-4, Jurkat, THP-1, HeLa, MCF7, A431 Ramos and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.3 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MCM7/PRL Antibody (Ab114573) - Western Blot All lanes: Recombinant MCM7/PRL Antibody (Ab114573) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa Observed band size: 82 kDa Exposure time: 8.8 s
Recombinant MCM7/PRL Antibody (Ab114573) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM/7PRL Antibody (Ab114573). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant MCM7/PRL Antibody (Ab114573) - IF/ICC
IF analysis of Recombinant MCM7/PRL Antibody (Ab114573) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MCM7/PRL Antibody (Ab114573) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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