Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.), CAS No.9026-12-4

CAS: 9026-12-4 Cat. No.: R128610 Molecular Weight: 11 kDa EC Number: 232-809-1

GRADE & PURITY EnzymoPure™, ≥300,000 units/mg protein

Accession #

P00651

Overview

Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Application

Ribonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications.

Specifications

Product Name

Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.), CAS No.9026-12-4

Synonyms

RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥300, 000 units/mg protein

Biochemical and Physiological Mechanisms

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2

Accession #

P00651

CAS

9026-12-4

Enzyme Commission Number

3.1.27.3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥300,000 units/mg protein

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

One Unit releases the equivalent of one A260 unit of acid-soluble products from yeast RNA in 15 minutes at 37°C, pH 7.5