T4 DNA Polymerase

Cat. No.: T665886
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 5.0 U/μL
Accession #
P04415
Expression system
E. coli
Bioactivity
Catalytic incorporation of 10nmol deoxyribonucleotides (dNTPs) into polynucleotides at 37 ℃ for 30 minutes. The required enzyme quantity is defined as one active unit.
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Size
USA
Germany (EU)*
Price
Qty
100U
T665886-100U
US Made to order · 2–4 wks ·
—
$59.90
500U
T665886-500U
US Made to order · 2–4 wks ·
—
$179.90
4×500U
T665886-4×500U
US Made to order · 2–4 wks ·
—
$519.90
Enter a quantity for the sizes you want to add.

Specifications

Product Name
T4 DNA Polymerase
Synonyms
T4DNAPolymerase | Eta2 | DNA-directed DNA polymerase
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, RNase free, Sterile, EnzymoPure™
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, RNase free, sterile, 5.0 U/μL
Biochemical and Physiological Mechanisms
Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.
Bioactivity
Catalytic incorporation of 10nmol deoxyribonucleotides (dNTPs) into polynucleotides at 37 ℃ for 30 minutes. The required enzyme quantity is defined as one active unit.
Accession #
Molecule Type
Enzyme
Product Line
Biological Products
Storage and Shipping
Concentration
5.0 U/μL
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (24 months). Avoid freeze/thaw cycle.

Advanced Data

Certificates (CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
PCR Laboratory Contamination Prevention and Efficient Reagent Application: —Optimizing Experimental Steps and Choosing
RNase Free Grade
RNase-Free Grade
Principles of Enzymology and Its Biotechnological Applications
DNA Polymerases: Structural and Functional Features, Key Types, and Application Guidance
A Functional Systems Review of Cellular Nucleic Acid Metabolism and Regulatory Enzymes in Gene Expression, RNA Homeostasis, and Immune Defense
Types, Properties, and Applications of Commonly Used Polymerases in Molecular Biology Experiments
Molecular Cloning Screening and Identification Technologies: Positive Clone Confirmation, Restriction Mapping, and Sequencing Validation
Mutant Library Construction, Screening, and Performance Optimization in Protein-Directed Evolution
Solution Calculators
Reviews

Customer Reviews

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