Alomfilimab (anti-ICOS) - Primary antibody, specific to ICOS, >95%, Human IgG1

CAS: 2378692-24-9 Cat. No.: Ab179101

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human, Mouse

Isotype

Human IgG1

Conjugation

Unconjugated

Overview

Alomfilimab (anti-ICOS) is a human immunoglobulin G1 (IgG1) kappa monoclonal antibody that recognizes inducible T-cell co-stimulator (ICOS; CD278), with potential immunomodulating and antineoplastic activities.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Specifications

Product Name

Alomfilimab (anti-ICOS) - Primary antibody, specific to ICOS, >95%, Human IgG1

Synonyms

Activation inducible lymphocyte immunomediatory molecule antibody | Activation-inducible lymphocyte immunomediatory molecule antibody | AILIM antibody | CD278 antibody | CD278 antigen antibody | CD28-related Protein-1 antibody | CRP1 antibody | CVID1 anti

Specifications & Purity

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Host species

Human

Specificity

ICOS

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Isotype

Human IgG1

Light Chain Type

kappa

SDS-PAGE

26.1 kDa (Light Chain) & 48.8 kDa (Heavy Chain), under reducing conditions; 177.6 kDa, under non-reducing conditions.

Purification Method

Protein A purified

Purity

>95%

Concentration

See COA

CAS

2378692-24-9

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: Alomfilimab (anti-ICOS) (Ab179101)

Alomfilimab (anti-ICOS) (Ab179101) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated Jurkat cells (24h) labelling ICOS (red) with Alomfilimab (anti-ICOS) (Ab179101). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Alomfilimab (anti-ICOS) (Ab179101)

Alomfilimab (anti-ICOS) (Ab179101) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated MOLT-4 cells (24h) labelling ICOS (red) with Alomfilimab (anti-ICOS) (Ab179101). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Alomfilimab (anti-ICOS) (Ab179101)

Alomfilimab (anti-ICOS) (Ab179101) - SEC
The purity of Alomfilimab (anti-ICOS) (Ab179101) is more than 95% verified by HPLC.

ELISA: Alomfilimab (anti-ICOS) (Ab179101)

Alomfilimab (anti-ICOS) (Ab179101) - ELISA
Immobilized Cynomolgus/Rhesus Macaque ICOS/CD278-his a at 2.0 μg/mL can bind Alomfilimab (anti-ICOS) (Ab179101) with the EC₅₀ of 11.35 ng/mL.

Associated Targets(Human)

ICOS Tbio Inducible T-cell costimulator (0 Activities)

Discover Target: ICOS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action