ATF-4 Antibody - Primary antibody, specific to ATF4, Rabbit IgG

Cat. No.: Ab089952

GRADE & PURITY Validated, ExactAb™, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human ATF-4 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

ATF-4 Antibody - Primary antibody, specific to ATF4, Rabbit IgG

Synonyms

activating transcription factor 4 (tax-responsive enhancer element B67) | Activating transcription factor 4 | ATF4 | cAMP-dependent transcription factor ATF-4 | cAMP-responsive element-binding protein 2 | CREB-2DNA-binding protein TAXREB67 | cyclic AMP-de

Specifications & Purity

ExactAb™, Validated, See COA

Biochemical and Physiological Mechanisms

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.

Host species

Rabbit

Specificity

ATF4

Immunogen

A synthetic peptide derived from human ATF4 (AA 1-220).

Positive Control

WB: HeLa and HEK293 cell lysates. IHC: Mouse kidney tissue. ICC/IF: 293 cells.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: ATF-4 Antibody (Ab089952)

ATF-4 Antibody (Ab089952) - Western Blot
All lanes: ATF-4 Antibody (Ab089952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 39 kDa
Observed band size: 54 kDa
Exposure time: 20.0 s

IF/ICC: ATF-4 Antibody (Ab089952)

ATF-4 Antibody (Ab089952) - IF/ICC
Immunofluorescence analysis of ATF4 in 293 using ATF-4 Antibody (Ab089952).

IHC: ATF-4 Antibody (Ab089952)

ATF-4 Antibody (Ab089952) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse kidney using ATF-4 Antibody (Ab089952). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

• Application: Dilution info
• IHC:

  1/100 - 1/200;

• IF/ICC:

  1/50-1/200 ;

• WB:

  1/1000 - 1/2000

Associated Targets(Human)

ATF4 Tbio Cyclic AMP-dependent transcription factor ATF-4 (0 Activities)

Discover Target: ATF4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)