GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.0.9 mg/mL
ExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ATP6V1D Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/4000 IHC: 1/50-1/100 Protein Function Relevance': ""ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
Specifications
Product Name
ATP6V1D Antibody - Primary antibody, specific to ATP6V1D, Rabbit IgG
Synonyms
ATP6M antibody | ATP6V1D antibody | V-ATPase 28 kDa accessory proteinantibody | V-ATPase subunit D antibody | V-type proton ATPase subunit D antibody | Vacuolar ATP synthase subunit D antibody | Vacuolar proton pump subunit D antibody | VATD antibody | VM
Specifications & Purity
ExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL
Host species
Rabbit
Specificity
ATP6V1D
Immunogen
Fusion protein of human ATP6V1D (AA 169-238).
Positive Control
WB: Mouse brain tissue lysate, NIH/3T3, C6, HeLa and HEK293 cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
0.9 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 113.8 s
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 17.7 s
ATP6V1D Antibody (Ab090177) - IHC Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer using ATP6V1D Antibody (Ab090177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
ATP6V1D Antibody (Ab090177) - IF/ICC
IF analysis of ATP6V1D Antibody (Ab090177) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ATP6V1D Antibody (Ab090177) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/4000
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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