CD3 Rat mAb (Biotin) - Primary antibody, specific to CD3, Rat IgG2b

Cat. No.: Ab185405

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

17A2

Conjugation

Biotin

Overview

Flow: ≤0.3 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD3 Rat mAb (Biotin) - Primary antibody, specific to CD3, Rat IgG2b

Synonyms

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

CD3

Immunogen

γδTCR-positive T-T hybridoma D1

Positive Control

Flow: Mouse splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD3 Rat mAb (Biotin) (Ab185405)

CD3 Rat mAb (Biotin) (Ab185405) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Rat mAb (Biotin) (Ab185405) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD3 Rat mAb (Biotin) (Ab185405)

CD3 Rat mAb (Biotin) (Ab185405) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD3 Rat mAb (Biotin) (Ab185405) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)

Discover Target: CD3G

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)