Recombinant CD3 Antibody (AF647) - Primary antibody, specific to CD3

Cat. No.: Ab213111

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P49A24

Conjugation

AF647

Overview

Recombinant CD3 Antibody, clone P49A24, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD3 Antibody (AF647) - Primary antibody, specific to CD3

Synonyms

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b

Host species

Rat

Specificity

CD3

Immunogen

γδTCR-positive T-T hybridoma D1

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD3 Antibody (AF647) (Ab213111)

Recombinant CD3 Antibody (AF647) (Ab213111) - Flow Cytometry
Two-color flow cytometric analysis of CD3 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF647) (Ab190071; Left panel) or Recombinant CD3 Antibody (AF647) (Ab213111; Right panel) at 0.3μg/test.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)

Discover Target: CD3G

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)