Preparation of paraffin sections (hematoxylin-eosin counterstaining method)
Paraffin section making can be used to (1) maintain the normal interrelationships between cells, and can retain the original appearance of cells better and for
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Preparation of antibodies recognizing specific tyrosine phosphorylated polypeptides
Antibodies against phosphopeptides recognize proteins that are only in the phosphorylated state. The antibody does not cross-react with unphosphorylated protein
T lymphocyte proliferation assay
This module introduces the mouse T lymphocyte proliferation assay. Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from the "Comp
Separation and application of lipid rafts
Lipid rafts are important in immunology because T-cell receptors and several other important T-cell signaling proteins are present in lipid rafts or are recruit
Detection of apoptosis and other forms of cell death
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Phenotyping of Human Monocytes/Macrophages
To provide a phenotypic assay for human monocytes/macrophages. Author: J.E. Colligan et al, Translator: Xuitao Cao et al. This experiment is from the "Comprehen
Detection of intracellular factors by flow cytometry
This unit describes the use of 4-color fluorescence to analyze the levels of IFN-7, IL-4 and IL-2 expressed in CD4+ T cells. (can be used to calculate T h l and
Immunofluorescence and cell separation
Flow cytometry is used to analyze membrane surface antigens and intracellular antigens expressed by cells, and is capable of specifically distinguishing differe
Selection and preparation of antigen-presenting cells
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".