Allele-specific PCR
Allele-specific PCR, also known as mismatch PCR, amplification refractory mutation system (ARMS), and mismatch amplification mutation assay (MAMA), is an altern
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Reverse transcription-polymerase chain reaction
Reverse transcription-polymerase chain reaction (RT-PCR) has improved the sensitivity of RNA detection by several orders of magnitude, making it possible to ana
Simple allele discrimination PCR
Simple allel-discriminating PCR, which is based on the principle of amplifi-cation refractory mutation system (ARMS), in which primers are designed to introduce
Allele-specific PCR with L-DNA labeling
L-DNA-labeled allele-specific PCR combines allele-specific PCR with L-DNA-labeled PCR.L-DNA-labeled PCR amplifies DNA with a sequence-determined L-DNA tag.L-DNA
PCR-Denaturing Gradient Gel Electrophoresis
PCR-denaturing gradient gel electrophoresis is the process of amplifying the target gene through PCR by adding a nucleotide chain of 30-50 GC bases to the 5' en
In situ PCR
In situ PCR (in situ PCR) is a PCR reaction performed in tissue cells, which combines the advantages of in situ hybridization with cellular localization ability
Preparation of protein gel electrophoresis gels
Protein gel electrophoresis is commonly used to (1) analyze molecular biology, genetics, and biochemistry (2) preparative techniques (3) partially purify molecu
mutagenicity test
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Protein purification experiments
Protein molecules of different sizes enter the colloidal filtration column and can be separated according to their molecular weight differences; it is a widely