GST-pulldown procedure
The use of recombinant technology to fuse a probe protein with GST (Glutathione S transferase), and affinity binding of the fusion protein to GTH (Glutathione)
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Gelatin zymography
Gelatin zymography can be used for (1) the determination of gelatinase A (MMP-2) and gelatinase B (MMP-9), (2) the determination of inhibitors of gelatinases, a
Monosaccharide fermentation test
The monosaccharide fermentation test can be used to (1) test microorganisms with different abilities to utilize various carbon sources and (2) various tests for
Determination of Selegiline and its Metabolites in Human Urine by GC/MS
This experiment is mainly used for doping control.
LC-MS screening process for completely unknown drugs and toxic compounds in urine
LC-MS analysis can be applied to (1) the detection of toxic substances in the body and (2) pharmaceutical research.
Subcellular localization analysis
The target proteins are labeled with specific marker proteins such as green fluorescent protein (GFP) or β-glucosidase (GUS), and their location is subsequentl
Qualitative Identification of Aminotransfer Reactions
Qualitative assays for aminotransfer reactions can be used to: detect the presence of aminotransferases.
Extraction, isolation and characterization of heptaphylloside and heptaphyllolactone from the skin of Qin Pei
Qinpi is the bark of Fraxinus Chinensis Poxb or F. rhynchophylla Hance or F. bungeana DC, which is bitter in flavor and slightly cold in nature. It is bitter in
Methods for the extraction of barmartine and the preparation of fenugreekin
Palmatine is a quaternary ammonium alkaloid that is soluble in water and polar organic solvents (e.g., methanol, ethanol, etc.) so it can be extracted with meth