Biochemistry Lab

The monosaccharide fermentation test can be used to (1) test microorganisms with different abilities to utilize various carbon sources and (2) various tests for

Gelatin zymography can be used for (1) the determination of gelatinase A (MMP-2) and gelatinase B (MMP-9), (2) the determination of inhibitors of gelatinases, a

Qualitative assays for aminotransfer reactions can be used to: detect the presence of aminotransferases.

Han Fangzhi is the root of Stephania tetrandra S. Mcore, a species of the genus Stephania in the family Fangzhiidae, which is an expectorant, antipyretic and an

Palmatine is a quaternary ammonium alkaloid that is soluble in water and polar organic solvents (e.g., methanol, ethanol, etc.) so it can be extracted with meth

Rutin (Rutin) is widely present in the plant world, has been found to contain rutin in at least 70 kinds of plants, such as tobacco, acacia flowers, buckwheat a

In plants Diosgenin is present in combination with glucose and rhamnose to form Dioscin. When extracting and separating, Dioscin is generally hydrolyzed into Di

The extraction and isolation of the free hydroxyanthraquinone components in Tiger Balm can be applied to (1) the preparation of drugs and (2) the study of pharm

Qinpi is the bark of Fraxinus Chinensis Poxb or F. rhynchophylla Hance or F. bungeana DC, which is bitter in flavor and slightly cold in nature. It is bitter in

Extraction and isolation of palmarosa anthelmintic alkaloids and experiments on the preparation of yanhuisuo ethanol can be used for: utilizing it to alleviate

Rhubarb is recorded in the Shennong Ben Cao Jing (Classic of the Materia Medica of the Divine Husbandman) and many other documents, and is used for draining the

A class of nitrogen-containing alkaline organic compounds found in nature (mainly in plants, but some are also found in animals) have alkaloid-like properties,

Tannins, also known as tannins, are a class of complex polyphenolic compounds found in plants. Tannins can be combined with proteins to form an insoluble precip

Cyanogenic glycosides are mainly glycosides with α-hydroxynitrile. This kind of glycoside is easy to be hydrolyzed by dilute acid and enzyme catalyzed hydrolys

Anthracene glycoside and alkali salt chromogenic reaction (Borntrager reaction, sublimation material face up, put under the microscope observation, can see most

Flavonoid glycosides are polyphenolic substances that belong to the flavonoid group. Flavonoids (also known as flavonoids) refers to a series of compounds consi

Volatile oils, also known as essential oils, in English for essential oils, is a class of aromatic odors present in plants, can be distilled with water vapor an

The molecule consists of an alcohol group or alcohol-like group (ligand, glycoside or glucoside) bound to a variable number of sugar molecules. If the ligand co

Quantitative determination of saponins can be carried out by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), colorimetric method

The fluid infusion or infusion refers to the preparation made by extracting herbs with suitable solvents, evaporating some or all of the solvents, and adjusting

Granules are dry granular preparations made of drugs or herbal extracts with suitable excipients or fine powder of herbs. Preparation process: treatment of raw

Solution-type liquid pharmaceuticals are liquid pharmaceuticals formed by dispersing small molecules of drugs in a solvent in a molecular or ionic (diameter of

Liquor refers to the medicinal herbs with distilled liquor extract made of clarified liquid preparations, generally prepared by impregnation, a few percolation

Glycosides, also known as ligands, are compounds formed when a sugar or a derivative of a sugar is joined to another type of non-sugar substance through the car

Glass milk can be used for the recovery of DNA, this method has a variety of uses, the secondary include: purification of DNA fragments from agarose gel aliquot

Using paper as a carrier, a liquid stationary phase (e.g., water, formamide, or others) is uniformly adsorbed on the paper, and a solvent that is not miscible w

Dispersions are dry powdery preparations of drugs or with suitable excipients made by crushing and uniform mixing for internal or topical use. Internal dispersi

In this experiment, sucrose was used as the substrate to determine the time course curve of the reaction between sucrase and the substrate, i.e., under the opti

Understand the blood glucose determination kit method and master the basic principles and methods of blood glucose enzymatic determination. (Source: Laboratory

Design your own experimental program to determine the effects of pH, temperature and inhibitors on sucrase activity. (Source: Laboratory instruction of biochemi

Combination of Chinese medicines refers to liquid preparations for internal use made of water or other solvents, extracted, purified and concentrated by suitabl

(Source: Laboratory instruction in biochemistry and molecular biology, Shao Xueling et al, Wuhan University Press, 2003)

Source of content: Tissue Culture and Molecular Cytology Techniques. (Beijing Publishing House)

Usually organochlorine pesticides (OCLs) are divided into DDT and its analogues, hexachlorohexane and cyclopentadienyl derivatives, which are not easy to be dec

The basic principle of the determination of glutamine-alpha transaminase activity; master the method of the determination of glutamine-alpha transaminase activi

In this experiment, polyacrylamide gel electrophoresis was used to analyze and compare the peroxide isoenzymes, and their activities were determined by guaiacol

Most mRNAs from mammalian cells have a poly(A) tail at the 3' end, so mRNA can be isolated from large amounts of cellular RNA by oligo(dT)-cellulose affinity ch

Source: Biochemistry and Molecular Biology Laboratory Guide, Shao Xueling et al, Wuhan University Press, 2003.

Heparin (Heparin) is a class of acidic mucopolysaccharides that are stable to alkali and heat, and exists in organisms as complexes bound to proteins, which are

Succinate dehydrogenase is an important enzyme in the tricarboxylic acid cycle, and the determination of the presence or absence of succinate dehydrogenase acti

Pyrethroids are an important class of synthetic insecticides with high efficiency, broad-spectrum, low toxicity and easy biodegradation. Source: Food Safety Tes

Residues in agricultural fields have also attracted the attention of agrochemists because some herbicides can cause damage to the following crop during applicat

Determination of residues of homotriazide herbicides and substituted urea herbicides in wheat to prevent some herbicides from causing damage to the next crop du

Urethane is generally non-corrosive and its storage stability is excellent. Its greatest advantage is that it remains in the plant for only a short time, and mo

Some herbicides can cause damage to the next crop during application resulting in reduced yields, so residues in agricultural fields have also attracted the att

In order to study the nature and role of food contaminating factors, some modern techniques are used to detect their levels in food, to determine the safe dose

Due to the requirement to detect more and more pesticide species, the limit is getting lower and lower, multi-residue detection technology has become the mainst

Macrolide antibiotics are antibiotics commonly used as veterinary drugs. There are many methods available for the determination of macrolide residues in animal

The main component of algal cell wall and extracellular polysaccharide is mucopolysaccharide, the monosaccharide composition of mucopolysaccharide of a part of

In recent years, regarding the detection technology of pesticide residues in food, the United States and the European Union have adopted more multi-residue dete

An aminoglycoside is a carbohydrate that contains one or more amino groups in its glycosidic structure. It is an antibiotic often used as a veterinary drug. Sou

The technique of determining the residues of epoetin, abamectin, doramectin and ivermectin in liver or muscle by SPE-HPLC-MS/MS is gradually maturing. Source: F

Some herbicides can cause damage to the next crop during application resulting in reduced yields, so residues in agricultural fields have also attracted the att

Quinolone residues in tissues are predominantly prodrugs, so prodrugs are generally chosen as indicator residues. Most of the metabolites are metabolized quickl

Hormone residues in foods of animal origin may jeopardize the health of consumers, with sex and growth-promoting hormones, which are currently the most used, po

LacZ fusion vectors were constructed to study yeast gene regulation. Because of the simplicity and sensitivity of this assay, yeast genes are often labeled with

Irradiation of protein-nucleic acid complexes with UV light induces covalent bond formation where nucleic acid nucleoproteins are in close contact, thus selecti

Thyroid inhibitors (antithyroida gents) are thioureas, which can be categorized into thioredoxins and imidazoles. Most of the early methods of purification trea

Most of the reported methods for the detection of nitrofuran residues still analyze the prodrugs. In recent years, research organizations have begun to focus on

Tetracyclines (TCs) antibiotics are produced by actinomycetes and all belong to hydrogenated and tetrabenzene ring derivatives in their chemical structure and h

There are many reported methods for the determination of chloramphenicol, and among the screening methods, GC-ECD and ELISA are more commonly used. source: Food

Myelin is a membrane that wraps around the axon of a nerve cell, i.e., myelin consists of the cell membrane of myelinating cells; current research pays attentio

Source : Practical Laboratory Techniques in Neurobiology

This presentation describes proteases that preferentially cleave Arg and Lys peptide bonds. Source: Laboratory Manual of Enzymology

Almost all neural cell cultures must have macromolecules as growth substrates to which the cells adhere in order to grow. Commonly used are polylysine and polyo

Source : Practical Laboratory Techniques in Neurobiology

Nucleic acid molecular chain is generally long, the use of ordinary drop or spray method is easy to make its structure damaged. Therefore, at present, most of t

Subchronic toxicity (subchronic toxicity) refers to the toxic effects that occur when experimental animals are exposed to a large dose of a foreign compound for

Source : Practical Laboratory Techniques in Neurobiology

Proteins are not directly immobilized to the substrate; spacer molecules can act as bridges connecting proteins to the substrate. Source: Laboratory Manual of E

This is an effective method for covalently immobilizing enzymes or proteins, such as peroxidase or antibodies, in agarose or cross-linked dextran. A serious dis

Source : Practical Laboratory Techniques in Neurobiology

Source : Practical Laboratory Techniques in Neurobiology

Source : Practical Laboratory Techniques in Neurobiology

Source : Practical Laboratory Techniques in Neurobiology

Source : Practical Laboratory Techniques in Neurobiology

This method is suitable for materials with a critical diol structure, such as cellulose, agarose, and dextran. Oxidation of periodate results in the formation o

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

This technique is more efficient and gentle compared to the chloride method. Source: Laboratory Manual of Enzymology

This method is representative of various cross-linking reactions and is one of the oldest and most practical. It allows the formation of intermolecular bridges

The resistance of glass to strong acids and solvents and its compact solid structure make it a valuable material for enzyme immobilization in technical processe

Antibodies or other proteins must have a free thiol group in order to obtain immobilized enzymatic activity. This group can be introduced by specific reagents,

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

absorbance

PPO activity assay can be applied to (1) study the physiological mechanism and regulation of PPO. (2) To understand the relationship between enzyme activity and

NADPH is a coenzyme called reduced coenzyme II, the scientific name of reduced nicotinamide adenine dinucleotide phosphate, once known as pyridine triphosphate

Dispersions are dry powdery preparations of drugs or with suitable excipients made by crushing and uniform mixing for internal or topical use. Internal dispersi

Cytochrome P450 (cytochromeP450 or CYP450, for short) is a superfamily of a class of ferrous heme-thiolate proteins that are involved in the metabolism of endog

Cytochrome b5 is a cytochrome deposited in the endoplasmic reticulum membrane of higher animals. It can be purified from rabbit liver microsomes. Because it is

China has made great progress in recent years in the series determination of many kinds of pesticide residues. Source: Food Safety Monitoring Technology (Chemic

This experiment takes the action of salivary amylase on starch at different temperatures and pH as an example to observe the effect of temperature and pH on the

In recent years, China has made great progress in the series of determination of various pesticide residues, and has issued four national standard methods. For

(Source: Laboratory instruction in biochemistry and molecular biology, Shao Xueling et al., Wuhan University Press, 2003)

If veterinary drugs are used inappropriately or without a prescribed rest period, they can lead to excessive residues of veterinary drugs in animal foods, and t

Sulfonamides (SAs) is a general term for a class of drugs with the structure of p-aminobenzenesulfonamide. They are widely used as chemotherapeutic drugs for th

Beta-agonists, chemically belonging to the phenylethanolamine group, are chemical analogs of catecholamines, epinephrine and norepinephrine. Source: Food Safety

Proteins synthesized in vitro are extremely useful for determining whether a cloned gene encodes a specific DNA-binding protein and for analyzing DNA-protein in

Detection of Firefly Luciferase Activity by Using Luciferin as a Substrate