Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The soils encountered by cleaning products are not all the same. Water-soluble stains, limescale, greasy soils, adhesive residues, resins, and waxy residues require different solvents and formulation systems. To determine whether a naturally derived solvent is suitable for a cleaning product, ...
Many stability problems in home and personal care products do not come only from the main active ingredients, surfactants, or fragrances themselves, but from an unsuitable solvent and solubilization system design. A transparent hand wash may become cloudy after fragrance is added; a serum may ...
Dimethyl sulfoxide (DMSO)
In the process of nuclear protein extraction, the use of surfactants can significantly interfere with protein labeling efficiency. This is because surfactants can alter the conformation of proteins and may interact with the target protein, affecting its biological activity and the accuracy of ...
L-Tyrosine is a crucial non-essential amino acid widely used in cell culture and various laboratory research applications. As a fundamental component of protein synthesis, L-Tyrosine not only participates in the synthesis of various biomolecules but also plays a vital role in cell development ...
Competent E. coli Cell Preparation Using the Calcium Chloride Method
Solid-phase peptide synthesis (SPPS) is a revolutionary biochemical technique that has become the primary method for synthesizing peptides and proteins since its introduction in the 1960s. Robert Bruce Merrifield first proposed the concept of SPPS in 1963, and this groundbreaking work not only ...
1, 4-dioxane, a heterocyclic organic ether, is a colorless liquid that emits a subtle, sweet aroma reminiscent of diethyl ether. Commonly referred to simply as dioxane due to the scarcity of its other isomers (1, 2- and 1, 3-), this compound finds widespread use as a solvent across numerous ...
In peptide synthesis, Boc protection groups are widely used to protect the amino terminus of amino acids to prevent unnecessary reactions during synthesis. After the peptide synthesis is complete, the peptide is released from the resin through the cleavage and deprotection steps, and these ...
Butan-2-ol, or sec-butanol, is an organic compound with formula CH3CH(OH)CH2CH3. Its structural isomers are 1-butanol, isobutanol, and tert-butanol. 2-Butanol is chiral and thus can be obtained as either of two stereoisomers designated as (R)-(−)-butan-2-ol and (S)-(+)-butan-2-ol. It is ...
Techniques often explored alongside immunological experiments.
