Fluorescent Mounting Media

Cat. No.: F598330

Overview

Anti fluorescence quencher is a reagent that can slow down fluorescence quenching. It can be used in most fluorescent dyes with simple operation. This product is recommended to be used in fixed permeabilized cells or tissues. After observation, nail polish or sealant can be used to seal the edge of the coverslip; DAPI containing antifluorescent quencher can directly stain nuclei.

Presentation：

Before using the product, return it to room temperature, slightly shake and mix to avoid bubbles.
1.Cell samples : 
( 1 ) After dyeing, absorb the liquid. ( 2 ) Add 10-20 μL anti-quenching agent to each glass slide, cover the cover glass with cells, and let the cells contact the anti-quenching agent. When the cover glass is covered, the excess can be squeezed out. 
The anti-quenching agent, try not to have bubbles. ( 3 ) Fluorescence microscope observation. ( 4 ) ( optional ) : After the observation, nail polish or sealant can be used to seal the edge of the cover glass. 
2. Tissue sections : 
( 1 ) After the staining is completed, the staining solution is sucked off. ( 2 ) Drop 10-20 μL of anti-quenching agent on the tissue section, cover the cover glass, let the section contact the anti-quenching agent, and squeeze out the excess anti-quenching agent when cover the cover glass to avoid bubbles as much as possible. ( 3 ) Observation of tissue sections by fluorescence microscope. ( 4 ) ( optional ) : After the observation, nail polish or sealant can be used to seal the edge of the cover glass. 
3.Other samples : Other samples can be operated with reference to the above samples.

Matters needing attention：

1. the anti fluorescence quencher has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes. It is recommended that the anti fluorescence quencher be used to fix permeabilized cells or tissues. 2. when the anti fluorescence quencher is matched with our YF 488 dye, there may be background interference. This situation may be caused by adding too much of this product. The amount of this product should be minimized. 3. the anti fluorescence quencher may not be suitable for some dyes. Pre experiment is recommended to test the matching before the experiment. 4. for your safety and health, please wear experimental clothes and disposable gloves.

Scope of application：

Fluorescent Mounting Media

Specifications

Product Name

Fluorescent Mounting Media

Molecule Type

Unknown

Storage and Shipping

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice