Experiments on preparation of DNA probes labeled with potenticin
Experiments on preparation of DNA probes labeled with potenticin
The digoxin system is over provided as another non-isotopic labeling method, which is detected by coupling an anti-digoxin antibody to one or more fluorescent dyes or enzymes, or by indirect immunofluorescence.
Operation method
Basic methods
Materials and Instruments
DNA Move 1. Establish a standard 100 μl reaction system with 10 μl of 10× digoxigenin-11-dUTP/dTTP reservoir, with no change in the amount of DNAzyme Ⅰ enzyme, and incubate at 15°C for 2 h. For more product details, please visit Aladdin Scientific website.
dTTP dUTP EDTA SDS
Water Bath Incubator
2. Take a small portion in microgel for electrophoresis to detect the probe size.
3. Continue the reaction until a DNA probe of appropriate size is obtained.4. Add 2 μl of 0.5 mol/l EDTA and 1 μl of 10% SDS and heat at 68°C for 10 min to terminate the reaction.
5. Separate the probe with a G-50 centrifugal column.6. Determination of digoxin-labeled probes by colorimetric or chemiluminescent methods.
7. Replacement of streptavidin-alkaline phosphatase cross-links with alkaline phosphatase-coupled anti-digoxin antibodies.
8. Detection of digoxigenin incorporation efficiency using digoxigenin-labeled labeled DNA or labeled digoxigenin probe as a control.