NDUFA9 Antibody - Primary antibody, specific to NDUFA9, Rabbit IgG

Cat. No.: Ab117346
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0 mg/mL
Application
IF/ICC, IHC, WB
Host species
Rabbit
Reactive species
Human, Mouse
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab117346-10μl
2
$99.90
50μl
Ab117346-50μl
1
$259.90
100μl
Ab117346-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab117346-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human NDUFA9 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.
Application:

WB: 1/500-1/1000 
IHC: 1/50-1/100

IF/ICC: 1/200
Protein Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Specifications

Product Name
NDUFA9 Antibody - Primary antibody, specific to NDUFA9, Rabbit IgG
Synonyms
CI-39kD antibody | Complex I subunit NDUFA9 antibody | Complex I-39kD antibody | mitochondrial antibody | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 antibody | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial an
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species
Rabbit
Specificity
NDUFA9
Immunogen
Fusion protein of human NDUFA9 (AA 11-152)
Positive Control
WB: A431, MCF7, HEK293, K562, Jurkat, Hep G2 and HeLa cell lysates. IHC: Human liver cancer tissue. IF./ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

NDUFA9 Antibody (Ab117346) - Western Blot
All lanes: NDUFA9 Antibody (Ab117346) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 34 kDa
Exposure time: 1.0 s

NDUFA9 Antibody (Ab117346) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NDUFA9 Antibody (Ab117346). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

NDUFA9 Antibody (Ab117346) - IF/ICC
IF analysis of NDUFA9 Antibody (Ab117346) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with NDUFA9 Antibody (Ab117346) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/200

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
NDUFA9 Tclin NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303450Certificate of AnalysisMar 20, 2024 Ab117346
ZJ24F0303449Certificate of AnalysisMar 20, 2024 Ab117346
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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