GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Prohibitin Antibody, Polyclonal, could be used for WB, IHC and so on. Application:
WB: 0.01-2 µg/mL IHC: 5-30 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Specifications
Product Name
Prohibitin Antibody - Primary antibody, specific to Prohibitin, Rabbit IgG
Synonyms
Epididymis luminal protein 215 antibody | Epididymis secretory sperm binding protein Li 54e antibody | HEL 215 antibody | HEL S 54e antibody | PHB antibody | PHB_HUMAN antibody | PHB1 antibody | Prohibitin antibody | PHB | PHB1 | Prohibitin | HEL-215 | HE
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Rabbit
Specificity
Prohibitin
Immunogen
Recombinant PHB expressed in E.coli (AA 177-272).
Positive Control
WB: HeLa, MCF7, Ramos, A431, HEK293, Hep G2, Jurkat, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, rectum, thyroid cancer, liver, liver cancer tissues; Mouse cardiac muscle tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified + Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Prohibitin Antibody (Ab123227) - Western Blot All lanes: Prohibitin Antibody (Ab123227) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 28 kDa Exposure time: 14.8 s
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Rectum Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Sample: Mouse Cardiac Muscle Tissue Primary Ab: 30 µg/mL Prohibitin Antibody (Ab123227) Control: Used PBS instead of primary antibody Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IF/ICC IF analysis of Prohibitin Antibody (Ab123227) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Prohibitin Antibody (Ab123227) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
5-30 µg/mL
WB
0.01-2 µg/mL
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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