Recombinant EGFR Antibody (Biotin) - Primary antibody, specific to ERBB1/EGFR/HER1

CAS: 205923-56-4 Cat. No.: Ab215355 Molecular Weight: 145543.35

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Human

Reactive species

Cynomolgus monkey, Human, Mouse

Isotype

Human IgG1

Conjugation

Biotin

Overview

Recombinant EGFR Antibody, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant EGFR Antibody (Biotin) - Primary antibody, specific to ERBB1/EGFR/HER1

Synonyms

C225 | Recombinant Cetuximab Antibody | Cetuximab (anti-EGFR) | Cetuximab (C225) | Anti-ERBB1 / EGFR / HER1 Reference Antibody (cetuximab) | Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell growth inhibiting protein 40 antibo

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Human

Specificity

ERBB1/EGFR/HER1

Positive Control

Flow Cytometry: A431

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

CAS

205923-56-4

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant EGFR Antibody (Biotin) (Ab215355)

Recombinant EGFR Antibody (Biotin) (Ab215355) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with Recombinant EGFR Antibody (Biotin) (Ab215355) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

EGFR Tclin Epidermal growth factor receptor (0 Activities)

Discover Target: EGFR

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

References

1. Li Jinbo, Yu Jiang, Song Jia, Zhang Yingxi, Li Ning, Wang Zhaomeng, Qin Meng, Zhao Mingming, Zhang Baoyue, Huang Ruiping, Zhou Shuang, Liu Yubo, He Zhonggui, Liu Hongzhuo, Dan Liu, Wang Yongjun.  (2025)  Galloylated liposomes enable targeted drug delivery by overcoming protein corona shielding.  Nature Communications,  16  (1): (1-15).  [PMID:40854890] [10.1038/s41467-025-63198-4]

2. Liyin Wang, Milad Ashrafizadeh, Gautam Sethi, Xinjia Zhou.  (2025)  pH-responsive biomimetic zeolitic imidazolate framework-based nanoparticles for co-delivery of cetuximab and siRNA in synergistic therapy of laryngeal squamous cell carcinoma.  Journal of Pharmaceutical Analysis,      [PMID:40735032] [10.1016/j.jpha.2025.101203]