TIGAR Antibody - Primary antibody, specific to TIGAR, Rabbit IgG

Cat. No.: Ab131228
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0 mg/mL
Application
IF/ICC, WB
Host species
Rabbit
Reactive species
Human, Mouse, Rat
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab131228-10μl
2
$99.90
50μl
Ab131228-50μl
1
$259.90
100μl
Ab131228-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab131228-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human TIGAR Antibody, Polyclonal, could be used for WB and so on.
Application:

WB: 1/500-1/2000

IF/ICC: 1/200
Protein Function
Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.

Specifications

Product Name
TIGAR Antibody - Primary antibody, specific to TIGAR, Rabbit IgG
Synonyms
EC 3.1.3.46 | TP53-induced glycolysis and apoptosis regulator | TP53-induced glycolysis regulatory phosphatase | 6-bisphosphatase TIGAR antibody | C12ORF5 antibody | chromosome 12 open reading frame 5 antibody | FR2BP antibody | Fructose-2, 6-bisphosphatas
Specifications & Purity
ExactAb™, Validated, 1.0 mg/mL
Host species
Rabbit
Specificity
TIGAR
Immunogen
A synthetic peptide derived from human TIGAR.
Positive Control
WB: HEK293, MCF7, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

TIGAR Antibody (Ab131228) - Western Blot
All lanes: TIGAR Antibody (Ab131228) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 26 kDa
Exposure time: 10.0 s

TIGAR Antibody (Ab131228) - IF/ICC
IF analysis of TIGAR Antibody (Ab131228) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with TIGAR Antibody (Ab131228) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
WB

1/500-1/2000

IF/ICC

1/200

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
TIGAR Tbio Fructose-2,6-bisphosphatase TIGAR (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606804Certificate of AnalysisJun 13, 2024 Ab131228
ZJ24F0606803Certificate of AnalysisJun 13, 2024 Ab131228
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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