GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human UBA1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/1000
IHC：1/100-1/500
IF/ICC: 1/200 Protein Function: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Specifications
Product Name
UBA1 Antibody - Primary antibody, specific to UBA1, Rabbit IgG
Synonyms
EC 6.2.1.45 | Protein A1S9 | Ubiquitin-activating enzyme E1 | A1S9 antibody | A1S9 protein antibody | A1S9T and BN75 temperature sensitivity complementing antibody | A1S9T antibody | A1ST antibody | AMCX1 antibody | CFAP124 antibody | CTD-2522E6.1 antibod
Specifications & Purity
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species
Rabbit
Specificity
UBA1
Immunogen
A KLH conjugated synthetic peptide from the N-terminal region of human UBA1 (AA 1-30)
Positive Control
WB: K562, HeLa, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified + Peptide affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
UBA1 Antibody (Ab100767) Western Blot All lanes: UBA1 Antibody (Ab100767) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 118 kDa Observed band size: 116 kDa Exposure time: 6.5 s
UBA1 Antibody (Ab100767) - IHC UBA1 Antibody (Ab100767) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UBA1 Antibody (Ab100767) for immunohistochemistry. Clinical relevance has not been evaluated.
UBA1 Antibody (Ab100767) - IF/ICC
IF analysis of UBA1 Antibody (Ab100767) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with UBA1 Antibody (Ab100767) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
1/100-1/500
WB
1/1000
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0303275
