GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on. Application WB:0.001-2 μg/mL IHC: 5-20 µg/mL Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Specifications
Product Name
alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1
Carrier Free, ExactAb™, Validated, High Performance, See COA
Especies hospedadoras
Mouse
Especificidad
Alpha smooth muscle Actin
Inmunógeno
Recombinant ACTA2 expressed in E.coli (AA 167-377).
Control positivo
WB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A + Protein G purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5.6 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 26.7 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Ovarian cancer Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Rectum Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Skeletal muscle Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Lung Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Pancreas Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Spleen Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0911292
