ExactAb™, Validated, Azide Free, High performance, 1.0mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human ACTB Antibody, Polyclonal antibody, could be used for WB, Flow, IHC, IF, ICC and so on.
Application
WB: 1/5000-1/ 50000
IHC: 1/ 500-1/ 1000
Flow: 1μg/Test
ICC/IF: 1/100
Protein Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Post-translational
ISGylated.
beta Actin Antibody (Ab091004) - Western Blot
All lanes: beta Actin Antibody (Ab091004) at 1/50000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Antibody (Ab091004) - IHC
Tissue: Human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded.
Antigen retrieval: Citrate buffer (0.01M, pH 6.0) boiling bathing for 15 minutes; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37℃ for 20 minutes.
Incubation: beta Actin Antibody (Ab091004) 1/1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
beta Actin Antibody (Ab091004) - ICC/IF
Hela cells: 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with beta Actin Antibody (Ab091004) 1/100, 90 minutes at 37°C; Followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.
| Aplicación | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |
| IHC |
1:500-1000
|
| IF/ICC |
1:100
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F0700610 | Certificate of Analysis | Apr 25, 2024 | Ab091004 |
| ZJ23F0700611 | Certificate of Analysis | Apr 25, 2024 | Ab091004 |
| ZJ24F0404301 | Certificate of Analysis | Apr 09, 2024 | Ab091004 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →
