CD154/CD40L Mouse mAb - Primary antibody, specific to TRAP/CD40L, Mouse IgG2a

Cat. No.: Ab132316
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
Animal Model, Blocking, Flow Cytometry, Functional Assay, IP
Host species
Mouse
Reactive species
Human, Monkey
Isotype
Mouse IgG2a
Clone Number
5C8
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab132316-10μg
5
49,90US$
50μg
Ab132316-50μg
2
139,90US$
100μg
Ab132316-100μg
1
209,90US$
1mg
Ab132316-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
1.399,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Mouse anti Human/Monkey CD154/CD40L Antibody, Monoclonal (5C8), could be used for in vitro blocking of CD40/CD40L signaling, in vivo blocking of CD40/CD40L signaling, IP, Flow and so on.

Application:
in vitro blocking of CD40/CD40L signaling: Use at an assay dependent concentration.

in vivo blocking of CD40/CD40L signaling: Use at an assay dependent concentration.

IP: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

Specifications

Product Name
CD154/CD40L Mouse mAb - Primary antibody, specific to TRAP/CD40L, Mouse IgG2a
Sinónimos
IGM | IMD3 | CD154 antigen | CD154 | CD40 antigen ligand | CD40 Ligand | CD40L | CD40-L | CD40LG | CD40LIGM | gp39 | hCD40L | HIGM1 | T-B cell-activating molecule | T-BAM | T-cell antigen Gp39 | TNF-related activation protein | TNFSF5 | TNFSF5IMD3 | TRAP
Especificaciones y pureza
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Especies hospedadoras
Mouse
Especificidad
TRAP/CD40L
Inmunógeno
Human D1.1 T cell line
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, PBS Only, Validated
Isotipo
Mouse IgG2a
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Imágenes

CD154/CD40L Mouse mAb (Ab132316) - ELISA
Immobilized Recombinant Human CD40 Ligand/TNFSF5 protein (rp189423) at 1.0 μg/mL can bind CD154/CD40L Mouse mAb (Ab132316) with the EC₅₀ of 3.301 ng/mL.

Aplicación
AplicaciónDilution info
IP

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vitro blocking of CD40/CD40L signaling

Use at an assay dependent concentration

in vivo blocking of CD40/CD40L signaling

Use at an assay dependent concentration

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
CD40LG Tbio CD40 ligand (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F1012611Certificate of AnalysisMay 20, 2026 Ab132316
ZJ24F1012612Certificate of AnalysisMay 20, 2026 Ab132316
ZJ24F1012613Certificate of AnalysisMay 20, 2026 Ab132316
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View Azide Free grade guide → View PBS Only grade guide →

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