ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human GABARAPL1 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application:
WB: 1/1000
IHC: 1/100-1/500
Flow: 1/10-1/50
Protein Function
Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Post-translational
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes. The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
GABARAPL1 Antibody (Ab104445) - Western Blot
All lanes: GABARAPL1 Antibody (Ab104445) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16, 18 kDa
Exposure time: 100.0 s
GABARAPL1 Antibody (Ab104445) - Flow
GABARAPL1 Antibody (Ab104445) flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
GABARAPL1 Antibody (Ab104445) - IHC
Formalin-fixed and paraffin-embedded human brain tissue reacted with GABARAPL1 Antibody (Ab104445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
| Aplicación | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB |
1/1000
|
| Flow Cytometry | 1/10-1/50 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ24F0202932 | Certificate of Analysis | Mar 01, 2024 | Ab104445 |
| ZJ24F0202933 | Certificate of Analysis | Mar 01, 2024 | Ab104445 |
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View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →
