Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti Human HDAC1 Antibody, Monoclonal (E22), could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 0.2-2μg/mL
IHC: 5-20μg/mL
ICC/IF: 5-20μg/mL
Protein Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
Post-translational
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
HDAC1 Mouse mAb (Ab107009) - Western Blot
All lanes: HDAC1 Mouse mAb (Ab107009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 63 kDa
Exposure time: 1.2 s
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Rat Colon Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Rat Testis Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Mouse Pancreas Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Mouse Cerebellum Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Aplicación | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.2-2 µg/mL
|
| IF/ICC |
5-20 µg/mL
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F0800907 | Certificate of Analysis | Aug 25, 2023 | Ab107009 |
| ZJ23F0800909 | Certificate of Analysis | Aug 25, 2023 | Ab107009 |
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