MX1 Antibody - Primary antibody, specific to MX1

Cat. No.: Ab116582

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Conjugation

Unconjugated

Descripción general

Rabbit anti Human MX1 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB: 0.5-5µg/mL

IHC: 5-20µg/mL

IF/ICC:  2µg/mL

Protein Function:

May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus. 

Specifications

Product Name

MX1 Antibody - Primary antibody, specific to MX1

Sinónimos

IFI-78K | Interferon-induced protein p78 | Interferon-regulated resistance GTP-binding protein MxA | Myxoma resistance protein 1 | Myxovirus resistance protein 1 | human interferon-regulated resistance GTP-binding protein MXA10IFI-78Khomolog of murine (in

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Especies hospedadoras

Rabbit

Especificidad

MX1

Inmunógeno

Recombinant MX1 expressed in E.coli (AA 80-342)

Control positivo

WB: Mouse lung, Mouse spleen, HeLa, A549 and C6 lysates. IHC: Human skin cancer, liver, breast cancer tissues. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Antigen affinity purified + Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - Western Blot
All lanes: MX1 Antibody (Ab116582) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 76 kDa
Observed band size: 78 kDa
Exposure time: 4.6 s

IHC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL MX1 Antibody (Ab116582)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL MX1 Antibody (Ab116582)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL MX1 Antibody (Ab116582)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IF/ICC  

IF analysis of MX1 Antibody (Ab116582) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MX1 Antibody (Ab116582) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.5-5 µg/mL ;

• IF/ICC:

  2 μg/mL;

Objetivos asociados (humanos)

MX1 Tbio Interferon-induced GTP-binding protein Mx1 (0 Activities)

Discover Target: MX1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)