Proteinase K, CAS No.39450-01-6

CAS: 39450-01-6 Cat. No.: P109033 Número EC: 254-457-8,3.4.21.64

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%, lyophilized powder,≥30 units/mg protein；expressed in Pichia pastoris

Accession #

P06873

Expression system

Pichia pastoris

Bioactivity

≥30 units/mg protein

Descripción general

This product is derived from Pichia pastoris expressing the Proteinase K gene from Parengyodontium album (formerly known as Tritirachium album Limber) optimized by site-directed mutagenesis. It exhibits advantages including high specific activity, high yield, and a broader active temperature and pH range.

Advanced protein engineering technology and a 28,000-liter yeast fermentation platform enable efficient unit cost control, providing cost-effective Proteinase K for biopharmaceutical R&D and production. High-efficiency yeast expression and chromatographic purification ensure product purity. The product is endotoxin-free, with a wider range of applications than native Proteinase K.

As one of the highly active proteases available, Proteinase K displays activity over a pH range of 4–12 and a temperature range of 0–75 °C, and retains activity even in the presence of SDS, urea, or EDTA. Proteinase K cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids and is capable of degrading virtually all proteins.

Product Characteristics

Source organism: Pichia pastoris expressing the genetically engineered Proteinase K gene from Parengyodontium album

Molecular weight: 29.3 kDa

pI: 8.9

Applications

Used in gene diagnostic kits, genomic DNA extraction kits, and RNA extraction kits to remove nucleases and other protein contaminants;

Used in the biopharmaceutical industry for the extraction of non-protein biological products such as nucleic acids and polysaccharide drugs, e.g., the preparation of DNA vaccines and heparin;

Used in skin care for the removal of aged keratinocytes, as well as in daily industrial fields including leather tanning, production of hydrolyzed protein seasonings, cosmetics, and feed processing.

Specifications

Product Name

Proteinase K, CAS No.39450-01-6

Sinónimos

(4-nitrophenyl)methyl (4R, 5S, 6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3, 4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate | BP178285 | AKOS032949645 | Endopeptidase K | Protease K | Endoproteinase K | Endopeptidase K | peptidase K |

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificaciones y pureza

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%, lyophilized powder, ≥30 units/mg protein；expressed in Pichia pastoris

Mecanismos bioquímicos y fisiológicos

Hydrolyzes keratin at aromatic and hydrophobic residues. Catalytic activity: Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides. Cofactor: Ca2+. Note: Binds 2 calcium ions per subunit.

Bioactividad

≥30 units/mg protein

Número de adhesión

P06873

Peso molecular previsto

29.3 kDa

CAS

39450-01-6

Número de la Comisión de Enzimas

EC 3.4.21.64

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

lyophilized powder,≥30 units/mg protein；expressed in Pichia pastoris

Reconstitución

Reconstitute in 20 mM Tris-HCl, 1 mM CaCl₂, pH 7.4.

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Definición de unidad

One unit is defined as the enzyme activity that produces 1 µmol of tyrosine per minute from casein at 37°C at pH 7.5.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Clase Lactams
      • Subclass Beta lactams

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Clase: Lactams
• Subclass: Beta lactams
• Intermediate Tree Nodes: Not available
• Direct Parent: Carbapenems
• Alternative Parents: Benzyloxycarbonyls Alpha amino acids and derivatives Nitrobenzenes Pyrroline carboxylic acids Phenoxy compounds Phenol ethers Nitroaromatic compounds Monoalkyl phosphates Azepines Alkyl aryl ethers Vinylogous esters Tertiary carboxylic acid amides Enoate esters Secondary alcohols Azetidines Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Monocarboxylic acids and derivatives Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Carbapenem - Benzyloxycarbonyl - Alpha-amino acid or derivatives - Nitrobenzene - Phenoxy compound - Nitroaromatic compound - Pyrroline carboxylic acid or derivatives - Pyrroline carboxylic acid - Phenol ether - Monoalkyl phosphate - Azepine - Alkyl aryl ether - Benzenoid - Alkyl phosphate - Phosphoric acid ester - Organic phosphoric acid derivative - Monocyclic benzene moiety - Vinylogous ester - Alpha,beta-unsaturated carboxylic ester - Enoate ester - Tertiary carboxylic acid amide - Pyrroline - Organic nitro compound - Secondary alcohol - C-nitro compound - Carboxylic acid ester - Carboxamide group - Azetidine - Azacycle - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Organic oxoazanium - Monocarboxylic acid or derivatives - Ether - Carboxylic acid derivative - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Carbonyl group - Alcohol - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as carbapenems. These are beta-lactam derivatives in which the beta-lactam ring shares the nitrogen atom with a pyrrole-2-carboxylic acid.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Referencias

1. Wenlong Pang, Bin Li, Yufeng Wu, Shaonan Tian, Yu Zhang, Jun Yang.  (2023)  Optimization of degradation behavior and conditions for the protease K of polylactic acid films by simulation.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:37858641] [10.1016/j.ijbiomac.2023.127496]

2. Kairui Duan, Nabila Mehwish, Mengdie Xu, Hu Zhu, Jiajun Hu, Mian Lin, Lu Yu, Bae Hoon Lee.  (2023)  Autoclavable Albumin-Based Cryogels with Uncompromising Properties.  Gels,  9  (9): (712).  [PMID:37754393] [10.3390/gels9090712]

3. Jie Zhang, Zhe Kong, Xiangyao Li, Chuang Zhang, Kaijun Xu, Guiqing Cheng, Zhongwei Wang, Qing Yu.  (2023)  The influence of Scutellaria baicalensis extract obtained from traditional Chinese herbal medicine on the thermo-oxidative degradation and stabilization of plasticized polylactic acid.  POLYMER ENGINEERING AND SCIENCE,  63  (9): (3105-3115).  [PMID:] [10.1002/pen.26431]

4. Zixuan Ding, Peilin Wang, Zhenrun Li, Yupeng Guo, Qiang Ma.  (2023)  Sulfur dots/Au@Ag nanorods array-based polarized ECL sensor for the detection of thyroid cancer biomarker.  TALANTA,      [PMID:37437396] [10.1016/j.talanta.2023.124925]

5. Qingxu Song, Gongde Lan, Qian Li, Yang Lin, Xin Chen, Hong Liu, Yufeng Cheng, Yuxia Luan.  (2023)  A hydrogel-enabled cascade amplification of radiosensitization and immune activation for cancer radiotherapy.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2023.142201]

6. Yanhua Xu, Huiyan Dong, Chenxi Liu, Haiwei Lou, Renyong Zhao.  (2023)  Efficient Aflatoxin B1 degradation by a novel isolate, Pseudomonas aeruginosa M-4.  FOOD CONTROL,      [PMID:] [10.1016/j.foodcont.2023.109679]

7. Lihuang Wu, Yuqi Wang, Xinyue Zhao, Hongli Mao, Zhongwei Gu.  (2023)  Investigating the Biodegradation Mechanism of Poly(trimethylene carbonate): Macrophage-Mediated Erosion by Secreting Lipase.  BIOMACROMOLECULES,      [PMID:36644840] [10.1021/acs.biomac.2c01350]

8. Guoli Xue, Yanjun Qu, Dan Wu, Shuyuan Huang, Yuqing Che, Jing Yu, Ping Song.  (2023)  Biodegradation of Aflatoxin B1 in the Baijiu Brewing Process by Bacillus cereus.  Toxins,  15  (1): (65).  [PMID:36668884] [10.3390/toxins15010065]

9. Xiangpeng Han, Mengyu Zhang, Jiayi Peng, Jinsong Wu, Qingping Zhong.  (2022)  Purification and characterization of a novel bacteriocin from Lactiplantibacillus plantarum Z057, and its antibacterial and antibiofilm activities against Vibrio parahaemolyticus.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2022.114358]

10. Tingting Wu, Xushun Liu, Hanjun Chen, Ying Liu, Yu Cao.  (2022)  An in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly and photonic crystals.  BIOSENSORS & BIOELECTRONICS,      [PMID:36529054] [10.1016/j.bios.2022.115013]

11. Mengdie Xu, Nabila Mehwish, Bae Hoon Lee.  (2022)  Facile Fabrication of Transparent and Opaque Albumin Methacryloyl Gels with Highly Improved Mechanical Properties and Controlled Pore Structures.  Gels,  8  (6): (367).  [PMID:35735711] [10.3390/gels8060367]

12. Fangjun Liu, Dun Wang, Jiaqi Wang, Liwei Ma, Cuiyun Yu, Hua Wei.  (2022)  Construction of Enzyme-Responsive Micelles Based on Theranostic Zwitterionic Conjugated Bottlebrush Copolymers with Brush-on-Brush Architecture for Cell Imaging and Anticancer Drug Delivery.  MOLECULES,  27  (9): (3016).  [PMID:35566368] [10.3390/molecules27093016]

13. Fangjun Liu, Dun Wang, Miao Zhang, Liwei Ma, Cui-Yun Yu, Hua Wei.  (2022)  Synthesis of enzyme-responsive theranostic amphiphilic conjugated bottlebrush copolymers for enhanced anticancer drug delivery.  Acta Biomaterialia,      [PMID:35306183] [10.1016/j.actbio.2022.03.028]

14. Xiaoping Wu, Qiujie Pang, Fuxiang Zhi, Xiaohong Mao, Yingbin Hu.  (2022)  Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells.  TISSUE & CELL,      [PMID:35325674] [10.1016/j.tice.2022.101769]

15. Tao Zhang, Dekun Zhang, Hongtao Liu, Kai Chen.  (2021)  Quantitative analysis and degradation mechanisms of different protein degradation methods.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,  110  (5): (1034-1043).  [PMID:34842354] [10.1002/jbm.b.34977]

16. Jing Li, Yong-Wei Feng, Li-Jun Huang, Rui Jiang, Xiao-Fang Shen.  (2021)  Strand-displacement DNA polymerase induced isothermal circular amplification fluorescence sensor for identification of pork component.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2021.101189]

17. Subramani Kandhasamy, Bo Liang, Da-Peng Yang, Yiming Zeng.  (2021)  Antibacterial Vitamin K3 Carnosine Peptide-Laden Silk Fibroin Electrospun Fibers for Improvement of Skin Wound Healing in Diabetic Rats.  ACS Applied Bio Materials,      [PMID:35007027] [10.1021/acsabm.0c01650]

18. Wei Yihua, Zhang Jinyan, Qiu Shuyan, Huang Qingqing, Yuan Linfeng, Chen Liumeng, Dai Tingcan, Tu Tianhua, Zhang Biaojin, Yan Han, Li Weihong.  (2021)  Selenium Species Determination in Se-Enriched Grain Crops with Foliar Spray of Sodium Selenite by IP-RP-HPLC-UV-HG-AFS.  Food Analytical Methods,  14  (7): (1345-1358).  [PMID:] [10.1007/s12161-021-01975-9]

19. Ying Wang, Xiaowen Xu, Xuan Zhao, Zongning Yin.  (2020)  Functionalized polymeric hybrid micelles as an efficient nanotheranostic agent for thrombus imaging and thrombolysis.  Acta Biomaterialia,      [PMID:33359293] [10.1016/j.actbio.2020.10.015]

20. Nan Yin, Ruihuan Zhao, Jie Zhang, Dingding Yang, Zhimin Guo, Rui Liu, Xin Yao.  (2020)  A lable-free SPR biosensor based on one peptide sequence with three recognition sites for O-GlcNAc transferase detection.  TALANTA,      [PMID:33167279] [10.1016/j.talanta.2020.121664]

21. Shao-Bin He, Paramasivam Balasubramanian, Ai-Ling Hu, Xiao-Qing Zheng, Meng-Ting Lin, Ming-Xuan Xiao, Hua-Ping Peng, Hao-Hua Deng, Wei Chen.  (2020)  One-pot cascade catalysis at neutral pH driven by CuO tandem nanozyme for ascorbic acid and alkaline phosphatase detection.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2020.128511]

22. Jianghui Qin, Shaoyin Wei, Hongyan Xu, Liwen Wang, Pengyan Hao, Bo Peng, Yagang Zhang, Pengzhong Shi, Xingjie Zan.  (2020)  The Bioinspired Facile Method to Efficiently Generate Diverse Proteinosomes with pH Switchable Permeability.  Advanced Materials Interfaces,  7  (14): (2000329).  [PMID:] [10.1002/admi.202000329]

23. Yufei Bian, Xuefei Leng, Zhiyong Wei, Zefeng Wang, Zhu Tu, Yanshai Wang, Yang Li.  (2019)  End-Chain Fluorescent Highly Branched Poly(l-lactide)s: Synthesis, Architecture-Dependence, and Fluorescent Visible Paclitaxel-Loaded Microspheres.  BIOMACROMOLECULES,      [PMID:31490668] [10.1021/acs.biomac.9b01020]

24. Chao Wang, Xiaodan Jia, Wenyao Zhen, Mengchao Zhang, Xiue Jiang.  (2019)  Small-Sized MOF-Constructed Multifunctional Diagnosis and Therapy Platform for Tumor.  ACS Biomaterials Science & Engineering,      [PMID:33438409] [10.1021/acsbiomaterials.9b00813]

25. Xuancheng Zhang, Zhaoyi Fang, Eunshinae Cho, Kai Huang, Jinzhong Zhao, Jia Jiang, Xiaoqiao Huangfu.  (2019)  Use of a Novel, Reinforced, Low Immunogenic, Porcine Small Intestine Submucosa Patch to Repair a Supraspinatus Tendon Defect in a Rabbit Model.  Biomed Research International,      [PMID:31073531] [10.1155/2019/9346567]

26. Zhi Li, He Zhang, Guohuai Li.  (2018)  Characterization of phytotoxin and secreted proteins identifies of Lasiodiplodia theobromae, causes of peach gummosis.  Fungal Biology,      [PMID:30654957] [10.1016/j.funbio.2018.11.001]

27. T. Cheng, X. Xu, W. Zhang, L. Chen, T. Liu.  (2017)  Protoplast preparation from enriched flagellates and resting cells of Haematococcus pluvialis.  JOURNAL OF APPLIED MICROBIOLOGY,  124  (2): (469-479).  [PMID:29154453] [10.1111/jam.13643]

28. Hao-Hua Deng, Xiu-Ling Lin, Yin-Huan Liu, Ke-Lin Li, Qiong-Qiong Zhuang, Hua-Ping Peng, Ai-Lin Liu, Xing-Hua Xia, Wei Chen.  (2017)  Chitosan-stabilized platinum nanoparticles as effective oxidase mimics for colorimetric detection of acid phosphatase.  Nanoscale,  9  (29): (10292-10300).  [PMID:28702672] [10.1039/C7NR03399K]

29. Zhifang Ma, Xiaolei Li, Xiaodan Jia, Jing Bai, Xiue Jiang.  (2016)  Folate-Conjugated Polylactic Acid–Silica Hybrid Nanoparticles as Degradable Carriers for Targeted Drug Delivery, On-Demand Release and Simultaneous Self-Clearance.  ChemPlusChem,  81  (7): (652-659).  [PMID:31968714] [10.1002/cplu.201600100]

30. Haibo Mu, Qianjin Liu, Hong Niu, Dongdong Wang, Jiangjiang Tang, Jinyou Duan.  (2015)  Autophagy promotes DNA–protein crosslink clearance.  MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,      [PMID:26921017] [10.1016/j.mrgentox.2015.12.001]

31. Zhifang Ma, Jing Bai, Xiue Jiang.  (2015)  Monitoring of the Enzymatic Degradation of Protein Corona and Evaluating the Accompanying Cytotoxicity of Nanoparticles.  ACS Applied Materials & Interfaces,      [PMID:26200209] [10.1021/acsami.5b05744]

32. Ying-Hui Shan, Li-Hua Peng, Xin Liu, Xi Chen, Jie Xiong, Jian-Qing Gao.  (2014)  Silk fibroin/gelatin electrospun nanofibrous dressing functionalized with astragaloside IV induces healing and anti-scar effects on burn wound.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,      [PMID:25556053] [10.1016/j.ijpharm.2014.12.067]

33. Ziyu Gan, Jun Wang.  (2025)  A MOF-stabilized enzyme-based colorimetric biosensor integrated with pH test strips for portable detection of D-limonene in agricultural applications.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.161719]

34. Wei Bao, Yunbao Gao, Jianing Zhang, Jing Jin, Na Zhang, Baijun Liu, Mingyao Zhang, Zhaohui Su, Hongwen Liang, Xiangling Ji, Wei Jiang.  (2025)  Biodegradable poly (lactic acid) blends toughened with in situ formed core-shell structure by reactive processing.  POLYMER,      [PMID:] [10.1016/j.polymer.2025.128330]

35. Xue Yunfan, Li Xiaoyan, Fan Zhuizhui, Ji Yating, Wang Jie, Chen Qinhu, Cai Zaisheng.  (2025)  Eco-friendly Anti-felting Finishing and Low-Temperature Dyeing of Wool Through Plant Protease.  FIBERS AND POLYMERS,      [PMID:] [10.1007/s12221-024-00838-4]

36. Weike Hu.  (2024)  EP300-mediated H3 acetylation elevates MTHFD2 expression to reduce mitochondrial dysfunction in lipopolysaccharide-induced tubular epithelial cells.  RENAL FAILURE,      [PMID:39230047] [10.1080/0886022X.2024.2369342]

37. Yu-Jun Shi, Ya-Ning Che, Yi-Mei Zhao, Rui-Xue Ran, Ya-Qi Zhao, Shi-Song Yu, Meng-Ying Chen, Lin-Yi Dong, Zhen-Yu Zhao, Xian-Hua Wang.  (2024)  High-efficient separation of deoxyribonucleic acid from pathogenic bacteria by hedgehog-inspired magnetic nanoparticles microextraction.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:38653039] [10.1016/j.chroma.2024.464923]

38. Peng Cui, Jiamin Wang, Lu Mao, Yuan Gao, Shuang Li, Yan Liu, Haimei Mao, Munan Qiu, Wenqi Zou, Xia Gao.  (2025)  Investigation of biodegradable microparticle release and environmental accumulation in Poly(butylene succinate) degradation under UV and non-UV aging conditions.  POLYMER DEGRADATION AND STABILITY,      [PMID:] [10.1016/j.polymdegradstab.2025.111323]

39. Yulong Wu, Jin Zhang, Ruikai Zhu, Hong Zhang, Dapeng Li, Huanhuan Li, Honggang Tang, Lihong Chen, Xinyan Peng, Xianrong Xu, Ke Zhao.  (2024)  Mechanistic Study of Novel Dipeptidyl Peptidase IV Inhibitory Peptides from Goat’s Milk Based on Peptidomics and In Silico Analysis.  Foods,  13  (8): (1194).  [PMID:38672866] [10.3390/foods13081194]

40. Yu Zhengtao, Xia Ying, Li Jiameng, Jiang Junwen, Li You, Li Youjun, Wang Liu.  (2024)  METTL3 mediates m6A modification of lncRNA CRNDE to promote ATG10 expression and improve brain ischemia/reperfusion injury through YTHDC1.  Biology Direct,  19  (1): (1-17).  [PMID:39407279] [10.1186/s13062-024-00536-4]

41. Jiajun Pan, Zhaopeng Xia, Haibao Zhang, Yang Lu, Yong Liu.  (2024)  One-step bleaching method for animal fiber by persulphate/hydrogen peroxide: Degradation mechanism of eumelanin.  Journal of Cleaner Production,      [PMID:] [10.1016/j.jclepro.2024.142256]

42. Wenlong Pang, Bin Li, Yufeng Wu, Yu Zhang, Jun Yang, Zhongxun Tian, Shaonan Tian, Jianjun Li.  (2024)  One-Step Synthesis of PtPb Alloy Nanoparticles Via Wet Chemical Method for the Upgraded Recycling of PLA Plastic.  ACS Sustainable Chemistry & Engineering,      [PMID:] [10.1021/acssuschemeng.4c06032]

43. Shuifang Mao, Yanming Ren, Yujun Zeng, Xingqian Ye, Jinhu Tian.  (2024)  pH-Responsive EGCG-Metal self-assembly as the carrier for rapid and prolonged insulin delivery.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2024.159048]

44. Ruopeng Yin, Tao Wang, Jingzu Sun, Huanqin Dai, Yuting Zhang, Ningning Liu, Hongwei Liu.  (2024)  Postbiotics From Lactobacillus Johnsonii Activates Gut Innate Immunity to Mitigate Alcohol-Associated Liver Disease.  Advanced Science,      [PMID:39574408] [10.1002/advs.202405781]

45. Shitong Wang, Kai Huang, Wenjing Shi, Jiajia Mo, Honghui Shi, Chuntai Liu, Baobao Chang.  (2024)  Promoting the formation of stereocomplex crystal in PLLA/PDLA by crosslinking restricted phase separation and solid-state stretching induced orientation.  POLYMER,      [PMID:] [10.1016/j.polymer.2024.127670]

46. Xiaofei Wang, Jili Han, Shuxian Zhang, Keshuai Liu, Xiaoxuan Fan, Changcai Bai, Guoning Chen.  (2024)  Self-polymerization silica nanoparticles based molecularly imprinted polymers for selective recognition of protein.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:39142168] [10.1016/j.chroma.2024.465260]

47. Kanzhen Liu, Cuiping Pang, Qinghua Li, Jianghua Li, Guocheng Du, Guoqiang Zhang.  (2025)  Step Enzymatic Hydrolysis and In Silico Screening-Assisted Preparation of Bioactive Peptides from Abalone.  Foods,  14  (7): (1209).  [PMID:40238341] [10.3390/foods14071209]

48. Cong Rui, Deng Chengyu, Li Pengwei, Tang Yiwei, Hou Jinfan, Zhao Jiehui, Wang Qili, Chen Yingying, Tu Jiasheng, Jiang Xiqun, Jiang Lei.  (2025)  Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing.  Nature Communications,  16  (1): (1-18).  [PMID:40592840] [10.1038/s41467-025-61141-1]

49. Shuming Liu, Chunhua Ge, Zhiqiang Li, Keke Chen, Xuefeng Li, Chengyao Peng, Xiangdong Zhang.  (2025)  High Thermal Conductivity and Biocompatibility Silk Fibroin Hydrogels Filled with CDs@BNOH for Body Surface Cooling.  ACS Applied Materials & Interfaces,      [PMID:40641287] [10.1021/acsami.5c09260]

50. Mao Wenqiang, Li Jiapeng, Wang Dongdong, Pan Yanxiong, Ji Xiangling, Jiang Wei.  (2025)  Biodegradable CPPC/PBS/PLA blends with enhanced adhesive properties by in situ compatibilization.  COLLOID AND POLYMER SCIENCE,      [PMID:] [10.1007/s00396-025-05476-7]

51. Yuchao Luo, Yingjie Hu, Tianyi Peng, Li Wen, Zikang Zhang, Ye Liu, Chengxin Chen, Yuxiang Wu, Zhihan Liu, Qingxia Liu, Zhixiang Chen.  (2025)  Self-charging, antibacterial, and biodegradable functional materials prepared by electrospinning: for masks, wound dressings, and vascular grafts.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.166917]

52. Xiaohai Bu, Liyi Chai, Yanmei Liu, Bing Feng, Tao Lu, Mingxin Feng, Yuming Zhou.  (2025)  Asymmetric hierarchically structured composite aerogels with enhanced dual-spectral selectivity for efficient radiative cooling.  COMPOSITES PART B-ENGINEERING,      [PMID:] [10.1016/j.compositesb.2025.112988]

53. Ranran Ma, Weiwei Peng, Yanli Xie, Nazish Muzaffar, Weibin Ma, Yuhui Yang, Qian Li, Hang Jia.  (2025)  Mechanistic Insights into Aflatoxin B1 and M1 Degradation by Bacillus subtilis HNGD-Mq02 from Wheat Koji and Its Application in Food Detoxification.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:40890944] [10.1021/acs.jafc.5c05089]

54. Hao Zhang, Yajin Guan, Meng Li, Yilin Wu, Xiaoou Sun.  (2025)  METTL3-Driven m6A Epigenetic Remodeling of lncRNA-AU020206 Stabilizes SLC7A11 via YTHDC2 Attenuates Apoptosis and Ferroptosis in Cerebral Ischemia/Reperfusion Injury.  Biomolecules,  15  (10): (1353).  [PMID:41154582] [10.3390/biom15101353]

55. Fuyou Xu, Zhenke Wen, Sidong Xiong.  (2025)  Immunogenic self-DNA drives neuropsychiatric systemic lupus erythematosus in conventional genetic background mice.  AUTOIMMUNITY,      [PMID:41029970] [10.1080/08916934.2025.2561610]

56. Yuan Chen, Meng Jiao Zhai, Nabila Mehwish, Meng Die Xu, Yi Wang, Yi Xuan Gong, Man Man Ren, Hui Deng, Bae Hoon Lee.  (2022)  Comparison of globular albumin methacryloyl and random-coil gelatin methacryloyl: Preparation, hydrogel properties, cell behaviors, and mineralization.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:35150780] [10.1016/j.ijbiomac.2022.02.028]

57. Hao-Hua Deng, Hua-Ping Peng, Kai-Yuan Huang, Shao-Bin He, Qiao-Feng Yuan, Zhen Lin, Rui-Ting Chen, Xing-Hua Xia, Wei Chen.  (2019)  Self-Referenced Ratiometric Detection of Sulfatase Activity with Dual-Emissive Urease-Encapsulated Gold Nanoclusters.  ACS Sensors,      [PMID:30652857] [10.1021/acssensors.8b01130]

58. Jun Huang, Gang Yang, Kewei Chen, Muying Du, Zsolt Zalán, Ferenc Hegyi, Jianquan Kan.  (2024)  Anti-fungal effects of lactic acid bacteria from pickles on the growth and sterigmatocystin production of Aspergillus versicolor.  INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,      [PMID:38955023] [10.1016/j.ijfoodmicro.2024.110809]

59. Jianjie Wu, Yuanyuan Zhang, Ping Sheng, Chunjiao He, Hao Liu, Shanyong Gu, Jiaoyang Luo, Meihua Yang.  (2025)  Ion-pairing reversed-phase chromatography with inductively coupled plasma-mass spectrometry applied to simultaneous determination of arsenic and selenium species in functional food American ginseng.  JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,      [PMID:40040335] [10.1002/jsfa.14211]

60. Shuming Liu, Chunhua Ge, Zhiqiang Li, Jinyao Shan, Keke Chen, Xuefeng Li, Yu Liu, Xiangdong Zhang.  (2024)  Visible-Light-Induced Silk Fibroin Hydrogels with Carbon Quantum Dots as Initiators for 3D Bioprinting Applications.  ACS Biomaterials Science & Engineering,      [PMID:39169444] [10.1021/acsbiomaterials.4c01189]

61. Ruikai Zhu, Hong Zhang, Yulong Wu, Huanhuan Li, Honggang Tang, Xianrong Xu, Ke Zhao.  (2025)  Exploration and molecular mechanism of novel ACE inhibitory peptides from goat milk protein: A combined in silico and in vitro study.  INTERNATIONAL DAIRY JOURNAL,      [PMID:] [10.1016/j.idairyj.2025.106265]

62. Yang Yang, Gengyu Zhou, Yang Chen, Ning Lin, Jingzhi Ma.  (2025)  Poly(lactide acid)-based microneedles enhanced by tunicate cellulose nanocrystals for potential diabetic periodontitis treatment.  CARBOHYDRATE POLYMERS,      [PMID:40368556] [10.1016/j.carbpol.2025.123629]

63. Dandan Feng, Zhu Zhu, Shangpeng Wang, Yangcan Gao, Yingwen Li, Yuying Sun, Jiquan Zhang.  (2025)  A type I crustin with an inhibitory effect on proteases and strong binding capacity to chitin from Neocaridina denticulata sinensis.  Comparative Immunology Reports,      [PMID:] [10.1016/j.cirep.2025.200226]

64. Jing Zhao, Maoning Li, Wenbang Liao, Yufei Shao, Qingyuan Du, Dandan Li, Yu Nan, Zhi Zhang, Dazhi Sun.  (2025)  Hierarchically porous degradable foam prepared by water-in-oil high internal phase emulsion template for high-performance recyclable oil - water separation.  JOURNAL OF HAZARDOUS MATERIALS,      [PMID:41207227] [10.1016/j.jhazmat.2025.140385]

65. Ling Ma, Shu-Ling Yang, Wen-Qian Lian, Xin-Feng Wei, Kai Zhang, Rui-Ying Bao, Wei Yang.  (2025)  Encapsulation of Enzymes in Metal–Organic Frameworks with Dual Thermal Protection for Melt Processing of Self-Biodegradable Plastics.  MACROMOLECULES,      [PMID:] [10.1021/acs.macromol.5c00584]

66. Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang.  (2025)  Octopod-shaped silver–gold alloy for NIR-driven synergistic photothermal and catalytic bacterial ablation.  COLLOIDS AND SURFACES B-BIOINTERFACES,      [PMID:41380240] [10.1016/j.colsurfb.2025.115353]

67. Zhen-Yu Zhao, Ya-Ning Che, Qian Huang, Han-Wen Yang, Xian-Hua Wang, Lin-Yi Dong.  (2025)  Rapid and sensitive detection of Candida albicans using nystatin-functionalized magnetic spheres.  JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,      [PMID:41447893] [10.1016/j.jchromb.2025.124896]

68. Nazish Muzaffar, Shujie Zhang, Qian Li, Yuhui Yang, Yanli Xie, Yuan Tian.  (2025)  Thermostable extracellular enzyme-mediated biodegradation of Aflatoxin B1 and Zearalenone in maize flour: A promising strategy.  INTERNATIONAL BIODETERIORATION & BIODEGRADATION,      [PMID:] [10.1016/j.ibiod.2025.106212]

69. Wanning Gao, Shengyuan Shi, Youcong Li, Fangyuan Liu, Yudi Cheng, Jie Zheng, Meng Qiao, Shuai Yuan, Xing Zhang.  (2026)  DNA-directed assembly of core-satellite MOF structures for spatially compartmentalized multi-enzyme cascade catalysis.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2026.173137]

70. Lu Tian, Baiqiang Chen, Jingxue Huang, Yu Cao, Tingting Wu.  (2026)  A multifunctional biomimetic interface biosensor for one-step amplified detection of tumor-derived exosomal miRNA.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2026.173463]

71. Han Pang, Qiang Zhao.  (2026)  Microscale thermophoresis sensor for homogeneous and one-step protein detection based on dual aptamers binding induced DNA switch.  TALANTA,      [PMID:41702041] [10.1016/j.talanta.2026.129535]

72. Dandan Feng, Yakun Song, Zuqi Wu, Wuruo Liu, Yuting Pu, Yangcan Gao, Yuying Sun, Jiquan Zhang.  (2026)  Physiological and biochemical characterization of trypsin from Neocaridina denticulata sinensis and its roles in ontogenesis and immune response.  PLoS One,  21  (2): (e0342746).  [PMID:41701695] [10.1371/journal.pone.0342746]

73. Xuan Ling, Ting Han, Pengfei Yang, Qian Zhang, Yong Xiang, Zhao Wang, Shikai Hu, Xiaoran Hu.  (2026)  Artificial Intelligence-Assisted Identification and Prevention of Chronic Obstructive Pulmonary Disease Using Biodegradable and Reusable Piezoelectric Elastomer/Poly(l-lactic acid) Composite Nanofiber Membrane.  ACS Applied Materials & Interfaces,      [PMID:41672635] [10.1021/acsami.5c23120]

74. Peng Wei, Junhui Li, Yuheng Chen, Jichao Zhang, Yuchen Liu, Yumin Xia, Yanping Wang.  (2026)  Comparative study the hydrolytic degradation behavior of high-performance biobased aromatic-aliphatic liquid crystal copolyesters in different degradation medium.  INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION,      [PMID:] [10.1080/1023666X.2025.2582797]

75. Lei Gong, Yuesheng Zhang, Enyang Xu, Hongtao Lu, Junrui Duan, Jianna Bao.  (2026)  Construction of Fully Biodegradable Poly(L-Lactide-Co-Caprolactone) Random Copolymer/Poly(D-Lactide) Blend: Crystallization, Toughness, and Biodegradability.  POLYMER ENGINEERING AND SCIENCE,      [PMID:] [10.1002/pen.70520]